biostudies-literatureBalouz VNational Institute of Allergy and Infectious DiseasesConsejo Nacional de Investigaciones Científicas y TécnicasMinisterio de Ciencia y TecnologíaNIAID NIH HHSNational Institutes of HealthNational Research Council214-225https://www.ebi.ac.uk/biostudies/studies/S-EPMC8900812biostudies-literatureUnknown37(3)Trypanosoma cruzi, the protozoan agent of Chagas' disease, displays a complex population structure made up of multiple strains showing a diverse ecoepidemiological distribution. Parasite genetic variability may be associated with disease outcome, hence stressing the need to develop methods for T. cruzi typing in vivo. Serological typing methods that exploit the presence of host antibodies raised against polymorphic parasite antigens emerge as an appealing approach to address this issue. These techniques are robust, simple, cost-effective, and are not curtailed by methodological/biological limitations intrinsic to available genotyping methods. Here, we critically assess the progress towards T. cruzi serotyping and discuss the opportunity provided by high-throughput immunomics to improve thiTrends in parasitologySerological Approaches for Trypanosoma cruzi Strain Typing.PMC8900812R01 AI123070Ricci ADAguero FBracco LBalouz VBuscaglia CARomer GfalseSerological Approaches for Trypanosoma cruzi Strain Typing.Trypanosoma cruzi, the protozoan agent of Chagas' disease, displays a complex population structure made up of multiple strains showing a diverse ecoepidemiological distribution. Parasite genetic variability may be associated with disease outcome, hence stressing the need to develop methods for T. cruzi typing in vivo. Serological typing methods that exploit the presence of host antibodies raised against polymorphic parasite antigens emerge as an appealing approach to address this issue. These techniques are robust, simple, cost-effective, and are not curtailed by methodological/biological limitations intrinsic to available genotyping methods. Here, we critically assess the progress towards T. cruzi serotyping and discuss the opportunity provided by high-throughput immunomics to improve thi2021-01-01T00:00:00Z2021 Mar2025-04-04T07:55:57.713Z2025-04-04T07:55:57.713ZS-EPMC89008123343631410.1016/j.pt.2020.12.002