biostudies-literatureUnknown12(1)Selmani ZBreast cancers expressing high levels of Ki67 are associated with poor outcomes. Oncotype DX test was designed for ER+/HER2- early-stage breast cancers to help adjuvant chemotherapy decision by providing a Recurrent Score (RS). RS measures the expression of 21 specific genes from tumor tissue, including Ki67. The primary aim of this study was to assess the agreement between Ki67_RNA obtained with Oncotype DX RS and Ki67_IHC. Other objectives were to analyze the association between the event free survival (EFS) and the expression level of Ki67_RNA; and association between RS and Ki67_RNA. Herein, we report a low agreement of 0.288 by Pearson correlation coefficient test between Ki67_IHC and Ki67_RNA in a cohort of 98 patients with early ER+/HER2- breast cancers. Moreover, Ki67_RNA^high tumors were significantly associated with the occurrence of events (p = 0.03). On the other hand, we did not find any association between Ki67_IHC and EFS (p = 0.26). We observed a low agreement between expression level of Ki67_RNA and Ki67 protein labelling by IHC. Unlike Ki67_IHC and independently of the RS, Ki67_RNA could have a prognostic value. It would be interesting to better assess the prognosis and predictive value of Ki67_RNA measured by qRT-PCR. The Ki67_RNA in medical routine could be a good support in countries where Oncotype DX is not accessible.Scientific reports3617https://www.ebi.ac.uk/biostudies/studies/S-EPMC8901910biostudies-literatureLow correlation between Ki67 assessed by qRT-PCR in Oncotype Dx score and Ki67 assessed by Immunohistochemistry.PMC8901910Curtit EDobi EFeugeas JPMeneveau NBazan FPivot XOvers ASelmani ZMeynard GAlgros MPMolimard CBorg CMansi LPaillard MJPretet JLViot JChaigneau LfalseLow correlation between Ki67 assessed by qRT-PCR in Oncotype Dx score and Ki67 assessed by Immunohistochemistry.Breast cancers expressing high levels of Ki67 are associated with poor outcomes. Oncotype DX test was designed for ER+/HER2- early-stage breast cancers to help adjuvant chemotherapy decision by providing a Recurrent Score (RS). RS measures the expression of 21 specific genes from tumor tissue, including Ki67. The primary aim of this study was to assess the agreement between Ki67_RNA obtained with Oncotype DX RS and Ki67_IHC. Other objectives were to analyze the association between the event free survival (EFS) and the expression level of Ki67_RNA; and association between RS and Ki67_RNA. Herein, we report a low agreement of 0.288 by Pearson correlation coefficient test between Ki67_IHC and Ki67_RNA in a cohort of 98 patients with early ER+/HER2- breast cancers. Moreover, Ki67_RNA^high tumors were significantly associated with the occurrence of events (p = 0.03). On the other hand, we did not find any association between Ki67_IHC and EFS (p = 0.26). We observed a low agreement between expression level of Ki67_RNA and Ki67 protein labelling by IHC. Unlike Ki67_IHC and independently of the RS, Ki67_RNA could have a prognostic value. It would be interesting to better assess the prognosis and predictive value of Ki67_RNA measured by qRT-PCR. The Ki67_RNA in medical routine could be a good support in countries where Oncotype DX is not accessible.2022-01-01T00:00:00Z2022 Mar2025-04-04T20:50:09.606Z2025-04-04T20:50:09.606ZS-EPMC89019103525665710.1038/s41598-022-07593-7