biostudies-literaturePal VKNational Institute of Environmental Health SciencesNIEHS NIH HHS151704https://www.ebi.ac.uk/biostudies/studies/S-EPMC8904290biostudies-literatureUnknown818Volatile organic compounds (VOCs) are ubiquitous environmental pollutants that are associated with birth defects, leukemia, neurocognitive deficits, reproductive impairment and cancer in humans exposed to these compounds. Exposure to VOCs can be assessed by measuring their metabolites in urine. Little is known, however, about the temporal variability in urinary VOC metabolite (VOCM) concentrations within- and between-individuals. In this study, we determined the variability in the concentrations of 38 VOCMs in urine samples collected from 19 healthy individuals across a period of 44 days. We also measured seven biomarkers of oxidative stress (lipid, protein and DNA damage) in urine to assess the relationship of VOC exposure to oxidative stress. Seventeen VOCMs had detection frequencies (DFThe Science of the total environmentDiurnal variability in urinary volatile organic compound metabolites and its association with oxidative stress biomarkers.PMC8904290U2C ES026542U2CES026542Li AJPal VKZhu HKannan KfalseDiurnal variability in urinary volatile organic compound metabolites and its association with oxidative stress biomarkers.Volatile organic compounds (VOCs) are ubiquitous environmental pollutants that are associated with birth defects, leukemia, neurocognitive deficits, reproductive impairment and cancer in humans exposed to these compounds. Exposure to VOCs can be assessed by measuring their metabolites in urine. Little is known, however, about the temporal variability in urinary VOC metabolite (VOCM) concentrations within- and between-individuals. In this study, we determined the variability in the concentrations of 38 VOCMs in urine samples collected from 19 healthy individuals across a period of 44 days. We also measured seven biomarkers of oxidative stress (lipid, protein and DNA damage) in urine to assess the relationship of VOC exposure to oxidative stress. Seventeen VOCMs had detection frequencies (DF2022-01-01T00:00:00Z2022 Apr2025-04-04T11:39:55.699Z2025-04-04T11:39:55.699ZS-EPMC89042903479380310.1016/j.scitotenv.2021.151704