{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liang C"],"funding":["Guangdong Innovative and Entrepreneurial Research Team Program","Foundation for the National Institutes of Health","National Natural Science Foundation of China","China Postdoctoral Science Foundation","NIGMS NIH HHS"],"pagination":["1217"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8904849"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["MicroRNAs (miRNAs) play crucial roles in gene expression regulation through RNA cleavage or translation repression. Here, we report the identification of an evolutionarily conserved WD40 domain protein as a player in miRNA biogenesis in Arabidopsis thaliana. A mutation in the REDUCTION IN BLEACHED VEIN AREA (RBV) gene encoding a WD40 domain protein led to the suppression of leaf bleaching caused by an artificial miRNA; the mutation also led to a global reduction in the accumulation of endogenous miRNAs. The nuclear protein RBV promotes the transcription of MIR genes into pri-miRNAs by enhancing the occupancy of RNA polymerase II (Pol II) at MIR gene promoters. RBV also promotes the loading of miRNAs into AGO1. In addition, RNA-seq revealed a global splicing defect in the mutant. Thus, this"],"journal":["Nature communications"],"pubmed_title":["Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading."],"pmcid":["PMC8904849"],"funding_grant_id":["GM129373","31870287","2014ZT05S078)","2015M582402","R01 GM129373","31701112"],"pubmed_authors":["Liang C","Mo B","Cai Q","Lan T","Li S","Gao L","You C","Cao D","Wang F","Xu C","Chen X","Zhang B"],"additional_accession":[]},"is_claimable":false,"name":"Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading.","description":"MicroRNAs (miRNAs) play crucial roles in gene expression regulation through RNA cleavage or translation repression. Here, we report the identification of an evolutionarily conserved WD40 domain protein as a player in miRNA biogenesis in Arabidopsis thaliana. A mutation in the REDUCTION IN BLEACHED VEIN AREA (RBV) gene encoding a WD40 domain protein led to the suppression of leaf bleaching caused by an artificial miRNA; the mutation also led to a global reduction in the accumulation of endogenous miRNAs. The nuclear protein RBV promotes the transcription of MIR genes into pri-miRNAs by enhancing the occupancy of RNA polymerase II (Pol II) at MIR gene promoters. RBV also promotes the loading of miRNAs into AGO1. In addition, RNA-seq revealed a global splicing defect in the mutant. Thus, this","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-19T13:02:17.011Z","creation":"2025-04-19T13:02:17.011Z"},"accession":"S-EPMC8904849","cross_references":{"pubmed":["35260568"],"doi":["10.1038/s41467-022-28872-x"]}}