{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ding Z"],"funding":["National Natural Science Foundation of China"],"pagination":["773671"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8905357"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["<h4>Purpose</h4>This study aimed to identify the biological functions of small intestine intestinal epithelial cell derived exosomes (IEC-Exos) and further distinguished the difference proteins in IEC-Exos between ileum and jejunum related to function of the digestive system and occurrence of several diseases.<h4>Materials and methods</h4>IECs of Male C57BL/6J mice were isolated. IEC-Exos were extracted from jejunum and ileum epithelial cell culture fluid by ultracentrifugation. In addition, isobaric tags for relative and absolute quantitation (iTRAQ) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to detect IEC-Exo proteins and conduct biological information analysis.<h4>Results</h4>The results showed that compared with jejunum IEC-Exos from ileum IEC-Exo"],"journal":["Frontiers in physiology"],"pubmed_title":["Unraveling the Proteomic Landscape of Intestinal Epithelial Cell-Derived Exosomes in Mice."],"pmcid":["PMC8905357"],"funding_grant_id":["81770542"],"pubmed_authors":["Li Q","Ding Z","Zhang C","Zhang B"],"additional_accession":[]},"is_claimable":false,"name":"Unraveling the Proteomic Landscape of Intestinal Epithelial Cell-Derived Exosomes in Mice.","description":"<h4>Purpose</h4>This study aimed to identify the biological functions of small intestine intestinal epithelial cell derived exosomes (IEC-Exos) and further distinguished the difference proteins in IEC-Exos between ileum and jejunum related to function of the digestive system and occurrence of several diseases.<h4>Materials and methods</h4>IECs of Male C57BL/6J mice were isolated. IEC-Exos were extracted from jejunum and ileum epithelial cell culture fluid by ultracentrifugation. In addition, isobaric tags for relative and absolute quantitation (iTRAQ) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to detect IEC-Exo proteins and conduct biological information analysis.<h4>Results</h4>The results showed that compared with jejunum IEC-Exos from ileum IEC-Exo","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-05-18T12:33:24.37Z","creation":"2025-05-18T12:33:24.37Z"},"accession":"S-EPMC8905357","cross_references":{"pubmed":["35283765"],"doi":["10.3389/fphys.2022.773671"]}}