biostudies-literatureDing ZNational Natural Science Foundation of China773671https://www.ebi.ac.uk/biostudies/studies/S-EPMC8905357biostudies-literatureUnknown13<h4>Purpose</h4>This study aimed to identify the biological functions of small intestine intestinal epithelial cell derived exosomes (IEC-Exos) and further distinguished the difference proteins in IEC-Exos between ileum and jejunum related to function of the digestive system and occurrence of several diseases.<h4>Materials and methods</h4>IECs of Male C57BL/6J mice were isolated. IEC-Exos were extracted from jejunum and ileum epithelial cell culture fluid by ultracentrifugation. In addition, isobaric tags for relative and absolute quantitation (iTRAQ) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to detect IEC-Exo proteins and conduct biological information analysis.<h4>Results</h4>The results showed that compared with jejunum IEC-Exos from ileum IEC-ExoFrontiers in physiologyUnraveling the Proteomic Landscape of Intestinal Epithelial Cell-Derived Exosomes in Mice.PMC890535781770542Li QDing ZZhang CZhang BfalseUnraveling the Proteomic Landscape of Intestinal Epithelial Cell-Derived Exosomes in Mice.<h4>Purpose</h4>This study aimed to identify the biological functions of small intestine intestinal epithelial cell derived exosomes (IEC-Exos) and further distinguished the difference proteins in IEC-Exos between ileum and jejunum related to function of the digestive system and occurrence of several diseases.<h4>Materials and methods</h4>IECs of Male C57BL/6J mice were isolated. IEC-Exos were extracted from jejunum and ileum epithelial cell culture fluid by ultracentrifugation. In addition, isobaric tags for relative and absolute quantitation (iTRAQ) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to detect IEC-Exo proteins and conduct biological information analysis.<h4>Results</h4>The results showed that compared with jejunum IEC-Exos from ileum IEC-Exo2022-01-01T00:00:00Z20222025-05-18T12:33:24.37Z2025-05-18T12:33:24.37ZS-EPMC89053573528376510.3389/fphys.2022.773671