biostudies-literatureUnknown12Pan P<i>Acinetobacter baumannii</i> is a type of bacterial nosocomial infection with severe drug resistance. Hemolysin co-regulated protein (Hcp) is a marker of activated type VI secretion system (T6SS), a key secretory system that promotes Gram-negative bacteria colonization, adhesion, and invasion of host cells. Hcp is also regulated by iron ions (Fe). In this study, an ATCC17978 <i>hcp</i> deletion strain (ATCC17978Δ<i>hcp</i>), an <i>hcp</i> complement strain (ATCC17978Δ<i>hcp⁺</i> ), and an <i>A. baumannii</i>-green fluorescent protein (GFP) strain were constructed and used to investigate the role of <i>hcp</i> in bacterial adhesion to cells (human pulmonary alveolar epithelial cells (HPAEpiC)) and biofilm formation. Our results indicate that the inhibitory concentrations of theFrontiers in cellular and infection microbiology761604https://www.ebi.ac.uk/biostudies/studies/S-EPMC8905654biostudies-literatureEffect of Hcp Iron Ion Regulation on the Interaction Between <i>Acinetobacter baumannii</i> With Human Pulmonary Alveolar Epithelial Cells and Biofilm Formation.PMC8905654Cheng TChen QYang YWei CPan PChen YYu DWang XWan HfalseEffect of Hcp Iron Ion Regulation on the Interaction Between <i>Acinetobacter baumannii</i> With Human Pulmonary Alveolar Epithelial Cells and Biofilm Formation.<i>Acinetobacter baumannii</i> is a type of bacterial nosocomial infection with severe drug resistance. Hemolysin co-regulated protein (Hcp) is a marker of activated type VI secretion system (T6SS), a key secretory system that promotes Gram-negative bacteria colonization, adhesion, and invasion of host cells. Hcp is also regulated by iron ions (Fe). In this study, an ATCC17978 <i>hcp</i> deletion strain (ATCC17978Δ<i>hcp</i>), an <i>hcp</i> complement strain (ATCC17978Δ<i>hcp⁺</i> ), and an <i>A. baumannii</i>-green fluorescent protein (GFP) strain were constructed and used to investigate the role of <i>hcp</i> in bacterial adhesion to cells (human pulmonary alveolar epithelial cells (HPAEpiC)) and biofilm formation. Our results indicate that the inhibitory concentrations of the2022-01-01T00:00:00Z20222026-05-31T07:06:06.984Z2024-11-12T19:09:41.002ZS-EPMC89056543528144510.3389/fcimb.2022.761604