biostudies-literatureGast VÅForskChalmers University of TechnologyVINNOVA36https://www.ebi.ac.uk/biostudies/studies/S-EPMC8905840biostudies-literatureUnknown21(1)<h4>Background</h4>Affibody molecules are synthetic peptides with a variety of therapeutic and diagnostic applications. To date, Affibody molecules have mainly been produced by the bacterial production host Escherichia coli. There is an interest in exploring alternative production hosts to identify potential improvements in terms of yield, ease of production and purification advantages. In this study, we evaluated the feasibility of Saccharomyces cerevisiae as a production chassis for this group of proteins.<h4>Results</h4>We examined the production of three different Affibody molecules in S. cerevisiae and found that these Affibody molecules were partially degraded. An albumin-binding domain, which may be attached to the Affibody molecules to increase their half-life, was identified to beMicrobial cell factoriesEngineering Saccharomyces cerevisiae for the production and secretion of Affibody molecules.PMC8905840(Cellnova) 2017-021052019-649Ekblad STous Mohedano MMolin MSiewers VSandegren AGast VEngqvist MKMThoren SDunas FGuler RfalseEngineering Saccharomyces cerevisiae for the production and secretion of Affibody molecules.<h4>Background</h4>Affibody molecules are synthetic peptides with a variety of therapeutic and diagnostic applications. To date, Affibody molecules have mainly been produced by the bacterial production host Escherichia coli. There is an interest in exploring alternative production hosts to identify potential improvements in terms of yield, ease of production and purification advantages. In this study, we evaluated the feasibility of Saccharomyces cerevisiae as a production chassis for this group of proteins.<h4>Results</h4>We examined the production of three different Affibody molecules in S. cerevisiae and found that these Affibody molecules were partially degraded. An albumin-binding domain, which may be attached to the Affibody molecules to increase their half-life, was identified to be2022-01-01T00:00:00Z2022 Mar2025-04-21T15:42:52.022Z2025-04-21T15:42:52.022ZS-EPMC89058403526415610.1186/s12934-022-01761-0