biostudies-literatureUnknown1Lau DCCanine Research FoundationFor the past 30 years, chemoprophylaxis with macrocyclic lactone (ML) anthelmintics has been the primary strategy for canine heartworm (<i>Dirofilaria immitis</i>) control in both the USA and Australia. ML-resistant <i>D. immitis</i> isolates have been confirmed to exist in the USA and studies have shown that 42 single nucleotide polymorphisms (SNPs) are associated with phenotypic ML-resistance. Currently, ML-resistance has not been reported in any Australian clinical cases of canine heartworm. The aim of the study is to determine whether the 42 SNPs associated with resistance to MLs in the isolates from the USA are present in adult heartworms from a clinical case in Australia. Five adult <i>D. immitis</i> obtained from a dog at post-mortem (Sydney, Australia) were sequenced using the IlluCurrent research in parasitology & vector-borne diseases100007https://www.ebi.ac.uk/biostudies/studies/S-EPMC8906102biostudies-literatureWhole-genome reference of <i>Dirofilaria immitis</i> from Australia to determine single nucleotide polymorphisms associated with macrocyclic lactone resistance in the USA.PMC8906102McLeod SLau DCCollaery STran ATPeou SLiang MSlapeta JfalseWhole-genome reference of <i>Dirofilaria immitis</i> from Australia to determine single nucleotide polymorphisms associated with macrocyclic lactone resistance in the USA.For the past 30 years, chemoprophylaxis with macrocyclic lactone (ML) anthelmintics has been the primary strategy for canine heartworm (<i>Dirofilaria immitis</i>) control in both the USA and Australia. ML-resistant <i>D. immitis</i> isolates have been confirmed to exist in the USA and studies have shown that 42 single nucleotide polymorphisms (SNPs) are associated with phenotypic ML-resistance. Currently, ML-resistance has not been reported in any Australian clinical cases of canine heartworm. The aim of the study is to determine whether the 42 SNPs associated with resistance to MLs in the isolates from the USA are present in adult heartworms from a clinical case in Australia. Five adult <i>D. immitis</i> obtained from a dog at post-mortem (Sydney, Australia) were sequenced using the Illu2021-01-01T00:00:00Z20212025-04-04T07:55:09.722Z2025-04-04T07:55:09.722ZS-EPMC89061023528487310.1016/j.crpvbd.2021.100007