biostudies-literatureChintala SNIDDK NIH HHSNational Institute of Diabetes and Digestive and Kidney DiseasesSchool of Pharmacy, University of Missouri-Kansas CityUniversity of Missouri1506-1514https://www.ebi.ac.uk/biostudies/studies/S-EPMC8906801biostudies-literatureUnknown7(4)In this work, we have brought the release of glucagon under the control of light. The aim of this approach is to allow minimally invasive, two-hormone control of blood glucose. Glucagon has two major challenges associated with its therapeutic application: (1) the required amount and timing of glucagon release is highly variable, and (2) glucagon rapidly fibrillates in solution, forming aggregates that are inactive. We have developed a light activated glucagon trimer, in which we have joined three glucagon molecules via light cleaved linkers. We demonstrated that this material can be stimulated by light to release glucagon in a predictable manner. In addition, we demonstrated that in the absence of light, the trimer does not form fibrils and thus releases normal unfibrillated glucagon upon ACS biomaterials science & engineeringA Light Activated Glucagon Trimer with Resistance to Fibrillation.PMC8906801DP3 DK106921R01DK123689R01 DK123689DP3DK106921Chintala SFriedman SHfalseA Light Activated Glucagon Trimer with Resistance to Fibrillation.In this work, we have brought the release of glucagon under the control of light. The aim of this approach is to allow minimally invasive, two-hormone control of blood glucose. Glucagon has two major challenges associated with its therapeutic application: (1) the required amount and timing of glucagon release is highly variable, and (2) glucagon rapidly fibrillates in solution, forming aggregates that are inactive. We have developed a light activated glucagon trimer, in which we have joined three glucagon molecules via light cleaved linkers. We demonstrated that this material can be stimulated by light to release glucagon in a predictable manner. In addition, we demonstrated that in the absence of light, the trimer does not form fibrils and thus releases normal unfibrillated glucagon upon 2021-01-01T00:00:00Z2021 Apr2025-04-19T09:22:52.052Z2025-04-19T09:22:52.052ZS-EPMC89068013370387410.1021/acsbiomaterials.1c00031