biostudies-literatureDalcin Martins PEuropean Research CouncilDutch Research Council (NWO)798906https://www.ebi.ac.uk/biostudies/studies/S-EPMC8906906biostudies-literatureUnknown13Microbial communities are key drivers of carbon, sulfur, and nitrogen cycling in coastal ecosystems, where they are subjected to dynamic shifts in substrate availability and exposure to toxic compounds. However, how these shifts affect microbial interactions and function is poorly understood. Unraveling such microbial community responses is key to understand their environmental distribution and resilience under current and future disturbances. Here, we used metagenomics and metatranscriptomics to investigate microbial community structure and transcriptional responses to prolonged ammonium deprivation, and sulfide and nitric oxide toxicity stresses in a controlled bioreactor system mimicking coastal sediment conditions. <i>Ca. Nitrobium versatile</i>, identified in this study as a sulfide-oFrontiers in microbiologyUnraveling Nitrogen, Sulfur, and Carbon Metabolic Pathways and Microbial Community Transcriptional Responses to Substrate Deprivation and Toxicity Stresses in a Bioreactor Mimicking Anoxic Brackish Coastal Sediment Conditions.PMC8906906MARIX 854088ALWOP.293024.002.002854088Op den Camp HJMOuboter HTJetten MSMDalcin Martins PEcheveste Medrano MJKurth JMWelte CUArshad AfalseUnraveling Nitrogen, Sulfur, and Carbon Metabolic Pathways and Microbial Community Transcriptional Responses to Substrate Deprivation and Toxicity Stresses in a Bioreactor Mimicking Anoxic Brackish Coastal Sediment Conditions.Microbial communities are key drivers of carbon, sulfur, and nitrogen cycling in coastal ecosystems, where they are subjected to dynamic shifts in substrate availability and exposure to toxic compounds. However, how these shifts affect microbial interactions and function is poorly understood. Unraveling such microbial community responses is key to understand their environmental distribution and resilience under current and future disturbances. Here, we used metagenomics and metatranscriptomics to investigate microbial community structure and transcriptional responses to prolonged ammonium deprivation, and sulfide and nitric oxide toxicity stresses in a controlled bioreactor system mimicking coastal sediment conditions. <i>Ca. Nitrobium versatile</i>, identified in this study as a sulfide-o2022-01-01T00:00:00Z20222026-06-01T02:36:34.807Z2024-10-16T15:04:59.997ZS-EPMC89069063528385710.3389/fmicb.2022.798906