{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wendler F"],"funding":["VPUE Course Development Grant","Juvenile Diabetes Research Foundation United States of America","Cancer Research UK","NIDDK NIH HHS","The Edward Penley Abraham Research Fund","John Fell Fund, University of Oxford","National Institutes of Health","National Institutes of Health,United States","Biotechnology and Biological Sciences Research Council"],"pagination":["3835"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8907290"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["The existence of three independent binary systems for conditional gene expression (Gal4/UAS; LexA/LexAop; QF/QUAS) has greatly expanded versatile genetic analyses in the Drosophila melanogaster; however, the experimental application of these tools is limited by the need to generate multiple collections of noninterchangeable transgenic fly strains for each inducible gene expression system. To address this practical limitation, we developed a modular vector that contains the regulatory elements from all three binary systems, enabling Gal4-, LexA- or QF-dependent expression of transgenes. Our methods also incorporate DNA elements that facilitate independent site-specific recombination and elimination of regulatory UAS, LexAop or QUAS modules with spatial and temporal control, thus offering un"],"journal":["Scientific reports"],"pubmed_title":["A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible Drosophila overexpression transgenes."],"pmcid":["PMC8907290"],"funding_grant_id":["162/001","P30 DK116074","C49979/A17516","U01 DK123743","BB/M011224/1","1757829","RF 290","R01 DK107507","R01 DK108817"],"pubmed_authors":["Sudarikova Y","Chang KR","Awan I","Liu S","Bustamante-Sequeiros M","Park S","Aisa-Bonoko G","Sung EY","Hill C","Wendler F","Baena-Lopez LA","Kim SK","Galasso A"],"additional_accession":[]},"is_claimable":false,"name":"A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible Drosophila overexpression transgenes.","description":"The existence of three independent binary systems for conditional gene expression (Gal4/UAS; LexA/LexAop; QF/QUAS) has greatly expanded versatile genetic analyses in the Drosophila melanogaster; however, the experimental application of these tools is limited by the need to generate multiple collections of noninterchangeable transgenic fly strains for each inducible gene expression system. To address this practical limitation, we developed a modular vector that contains the regulatory elements from all three binary systems, enabling Gal4-, LexA- or QF-dependent expression of transgenes. Our methods also incorporate DNA elements that facilitate independent site-specific recombination and elimination of regulatory UAS, LexAop or QUAS modules with spatial and temporal control, thus offering un","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-04T20:23:19.426Z","creation":"2025-04-04T20:23:19.426Z"},"accession":"S-EPMC8907290","cross_references":{"pubmed":["35264662"],"doi":["10.1038/s41598-022-07852-7"]}}