biostudies-literatureUnknown9Kumawat AKThe balance between pro- and anti-inflammatory cytokines released by immune and non-immune cells plays a decisive role in the progression of atherosclerosis. Interleukin (IL)-17A has been shown to accelerate atherosclerosis. In this study, we investigated the effect on pro-inflammatory mediators and atherosclerosis development of an Affibody molecule that targets IL17A. Affibody molecule neutralizing IL17A, or sham were administered <i>in vitro</i> to human aortic smooth muscle cells (HAoSMCs) and murine NIH/3T3 fibroblasts and <i>in vivo</i> to atherosclerosis-prone, hyperlipidaemic ApoE^-/- mice. Levels of mediators of inflammation and development of atherosclerosis were compared between treatments. Exposure of human smooth muscle cells and murine NIH/3T3 fibroblasts <i>in vitrFrontiers in cardiovascular medicine831039https://www.ebi.ac.uk/biostudies/studies/S-EPMC8907570biostudies-literatureInhibition of IL17A Using an Affibody Molecule Attenuates Inflammation in ApoE-Deficient Mice.PMC8907570Soderstrom LACaravaca ASSirsjo ABaumgartner RGistera AKetelhuth DFJHellberg SZegeye MMJin HLjungberg LUFrejd FYAmegavie OKumawat AKGudmundsdotter LOlofsson PSParamel GVfalseInhibition of IL17A Using an Affibody Molecule Attenuates Inflammation in ApoE-Deficient Mice.The balance between pro- and anti-inflammatory cytokines released by immune and non-immune cells plays a decisive role in the progression of atherosclerosis. Interleukin (IL)-17A has been shown to accelerate atherosclerosis. In this study, we investigated the effect on pro-inflammatory mediators and atherosclerosis development of an Affibody molecule that targets IL17A. Affibody molecule neutralizing IL17A, or sham were administered <i>in vitro</i> to human aortic smooth muscle cells (HAoSMCs) and murine NIH/3T3 fibroblasts and <i>in vivo</i> to atherosclerosis-prone, hyperlipidaemic ApoE^-/- mice. Levels of mediators of inflammation and development of atherosclerosis were compared between treatments. Exposure of human smooth muscle cells and murine NIH/3T3 fibroblasts <i>in vitr2022-01-01T00:00:00Z20222025-04-25T23:02:08.987Z2025-04-06T09:13:56.031ZS-EPMC89075703528236510.3389/fcvm.2022.831039