biostudies-literatureUnknown14(4)Cao HTumor-associated macrophages (TAMs) are critical immune cells infiltrated into tumor. In present study, we evaluated the effects of Qi Ling (QL), a traditional Chinese medicine on paclitaxel resistance in prostate cancer cells and explored the underlying mechanisms. We administrated QL to rats and collected the serum from QL-treated rats (QL-serum). We established the co-culture system of TAMs/paclitaxel resistant prostate cancer cells. We treated the TAMs with QL-serum and measured the viability of paclitaxel resistant prostate cancer cells after exposing to paclitaxel. We monitored the expression of M1 and M2 markers, the expression and activation of IL-6/STAT3 signaling pathways in TAMs after QL treatment. We treated TAMs with QL-serum together with interleukin (IL)-6, measured the exprAging1812-1821https://www.ebi.ac.uk/biostudies/studies/S-EPMC8908933biostudies-literatureQi Ling decreases paclitaxel resistance in the human prostate cancer by reversing tumor-associated macrophages function.PMC8908933Gao RCao HWang DFeng YChen LfalseQi Ling decreases paclitaxel resistance in the human prostate cancer by reversing tumor-associated macrophages function.Tumor-associated macrophages (TAMs) are critical immune cells infiltrated into tumor. In present study, we evaluated the effects of Qi Ling (QL), a traditional Chinese medicine on paclitaxel resistance in prostate cancer cells and explored the underlying mechanisms. We administrated QL to rats and collected the serum from QL-treated rats (QL-serum). We established the co-culture system of TAMs/paclitaxel resistant prostate cancer cells. We treated the TAMs with QL-serum and measured the viability of paclitaxel resistant prostate cancer cells after exposing to paclitaxel. We monitored the expression of M1 and M2 markers, the expression and activation of IL-6/STAT3 signaling pathways in TAMs after QL treatment. We treated TAMs with QL-serum together with interleukin (IL)-6, measured the expr2022-01-01T00:00:00Z2022 Feb2025-04-26T04:49:22.766Z2025-04-06T11:14:17.165ZS-EPMC89089333519398610.18632/aging.203904