biostudies-literatureUnknown38(1)Li Y<h4>Background</h4>Multiple myeloma remains a virtually incurable hematologic malignancy, which is featured with the aberrant growth of malignant plasma cells.<h4>Aims</h4>To elucidate the functions of miR-19a-3p in multiple myeloma.<h4>Study design</h4>Cell study.<h4>Methods</h4>Cell counting kit-8 assay was performed to detect cell viability, and flow cytometry was conducted to detect cell apoptosis. Bioinformatics analysis predicted miR-19a-3p-associated biological function, pathway, core regulatory network, and target genes. Luciferase reporter assay verified the target sequence of miR-19a-3p regulating FBXO32.<h4>Results</h4>miR-19a-3p is upregulated in multiple myeloma cells (p<0.01) and patients with multiple myeloma (p<0.001). Overexpressed miR-19a-3p significantly increased cell viability (p<0.05) and inhibited cell apoptosis (p<0.01). FBXO32 is a target gene of miR-19a-3p (p<0.01). Moreover, FBXO32 is downregulated in MM, and it significantly decreased cell viability (p<0.05) and promoted cell apoptosis (p<0.01). FBXO32 significantly rescued the influence of miR-19a-3p-inhibiting cell apoptosis (p<0.05).<h4>Conclusion</h4>miR-19a-3p promoted cell proliferation and inhibited cell apoptosis by degrading the target FBXO32 mRNA in multiple myeloma.Balkan medical journal43-49https://www.ebi.ac.uk/biostudies/studies/S-EPMC8909224biostudies-literaturemiR-19a-3p Functions as an Oncogene by Regulating FBXO32 Expression in Multiple Myeloma.PMC8909224Xue WLi YZhang DMa YMeng YGao SfalsemiR-19a-3p Functions as an Oncogene by Regulating FBXO32 Expression in Multiple Myeloma.<h4>Background</h4>Multiple myeloma remains a virtually incurable hematologic malignancy, which is featured with the aberrant growth of malignant plasma cells.<h4>Aims</h4>To elucidate the functions of miR-19a-3p in multiple myeloma.<h4>Study design</h4>Cell study.<h4>Methods</h4>Cell counting kit-8 assay was performed to detect cell viability, and flow cytometry was conducted to detect cell apoptosis. Bioinformatics analysis predicted miR-19a-3p-associated biological function, pathway, core regulatory network, and target genes. Luciferase reporter assay verified the target sequence of miR-19a-3p regulating FBXO32.<h4>Results</h4>miR-19a-3p is upregulated in multiple myeloma cells (p<0.01) and patients with multiple myeloma (p<0.001). Overexpressed miR-19a-3p significantly increased cell viability (p<0.05) and inhibited cell apoptosis (p<0.01). FBXO32 is a target gene of miR-19a-3p (p<0.01). Moreover, FBXO32 is downregulated in MM, and it significantly decreased cell viability (p<0.05) and promoted cell apoptosis (p<0.01). FBXO32 significantly rescued the influence of miR-19a-3p-inhibiting cell apoptosis (p<0.05).<h4>Conclusion</h4>miR-19a-3p promoted cell proliferation and inhibited cell apoptosis by degrading the target FBXO32 mRNA in multiple myeloma.2021-01-01T00:00:00Z2021 Jan2025-04-18T12:16:58.68Z2025-04-06T21:51:00.461ZS-EPMC89092243297551910.4274/balkanmedj.galenos.2020.2020.3.121