{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bi H"],"funding":["Scientific Research Project of Jingchu University of Technology","Natural Science Foundation of Hubei Province of China","National Natural Science Foundation of China","Scientific Research Program Guiding Project of Hubei Provincial Department of Education"],"pagination":["2784"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8911409"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(5)"],"pubmed_abstract":["Environmental stresses, especially heat and drought, severely limit plant growth and negatively affect wheat yield and quality worldwide. Heat shock factors (Hsfs) play a central role in regulating plant responses to various stresses. In this study, the wheat heat shock factor gene <i>TaHsfA2e-5D</i> on chromosome 5D was isolated and functionally characterized, with the goal of investigating its role in responses to heat and drought stresses. Gene expression profiling showed that <i>TaHsfA2e-5D</i> was expressed constitutively in various wheat tissues, most highly in roots at the reproductive stage. The expression of <i>TaHsfA2e-5D</i> was highly up-regulated in wheat seedlings by heat, cold, drought, high salinity, and multiple phytohormones. The TaHsfA2e-5D protein was localized in the nucleus and showed a transcriptional activation activity. Ectopic expression of the <i>TaHsfA2e-5D</i> in yeast exhibited improved thermotolerance. Overexpression of the <i>TaHsfA2e-5D</i> in <i>Arabidopsis</i> results in enhanced tolerance to heat and drought stresses. Furthermore, RT-qPCR analyses revealed that <i>TaHsfA2e-5D</i> functions through increasing the expression of Hsp genes and other stress-related genes, including <i>APX2</i> and <i>GolS1</i>. Collectively, these results suggest that <i>TaHsfA2e-5D</i> functions as a positive regulator of plants' responses to heat and drought stresses, which may be of great significance for understanding and improving environmental stress tolerance in crops."],"journal":["International journal of molecular sciences"],"pubmed_title":["Characterization of the Wheat Heat Shock Factor TaHsfA2e-5D Conferring Heat and Drought Tolerance in <i>Arabidopsis</i>."],"pmcid":["PMC8911409"],"funding_grant_id":["2019CFB296","B2018233","32072062 and 32001546","QDJ201901"],"pubmed_authors":["Bi H","Li H","Chen Q","Qian J","He J","Tian X","Ma D","Miao J","Zhao Y","Xu Y","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of the Wheat Heat Shock Factor TaHsfA2e-5D Conferring Heat and Drought Tolerance in <i>Arabidopsis</i>.","description":"Environmental stresses, especially heat and drought, severely limit plant growth and negatively affect wheat yield and quality worldwide. Heat shock factors (Hsfs) play a central role in regulating plant responses to various stresses. In this study, the wheat heat shock factor gene <i>TaHsfA2e-5D</i> on chromosome 5D was isolated and functionally characterized, with the goal of investigating its role in responses to heat and drought stresses. Gene expression profiling showed that <i>TaHsfA2e-5D</i> was expressed constitutively in various wheat tissues, most highly in roots at the reproductive stage. The expression of <i>TaHsfA2e-5D</i> was highly up-regulated in wheat seedlings by heat, cold, drought, high salinity, and multiple phytohormones. The TaHsfA2e-5D protein was localized in the nucleus and showed a transcriptional activation activity. Ectopic expression of the <i>TaHsfA2e-5D</i> in yeast exhibited improved thermotolerance. Overexpression of the <i>TaHsfA2e-5D</i> in <i>Arabidopsis</i> results in enhanced tolerance to heat and drought stresses. Furthermore, RT-qPCR analyses revealed that <i>TaHsfA2e-5D</i> functions through increasing the expression of Hsp genes and other stress-related genes, including <i>APX2</i> and <i>GolS1</i>. Collectively, these results suggest that <i>TaHsfA2e-5D</i> functions as a positive regulator of plants' responses to heat and drought stresses, which may be of great significance for understanding and improving environmental stress tolerance in crops.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-04-08T17:36:04.158Z","creation":"2025-04-04T22:09:00.807Z"},"accession":"S-EPMC8911409","cross_references":{"pubmed":["35269925"],"doi":["10.3390/ijms23052784"]}}