{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["43(5)"],"submitter":["Dahl KH"],"pubmed_abstract":["Molecular analysis of 17 genomically unrelated clinical VanB-type vancomycin-resistant enterococcus isolates from hospital patients in Germany, Norway, Sweden, the United Kingdom and the United States revealed three subtypes of the vanB gene cluster-vanB1, vanB2, and vanB3-which was in accordance with previous subtyping of the ligase gene sequence. There was no correlation between vanB subtype and levels of vancomycin resistance. All strains studied carried a structurally conserved vanB gene cluster as shown by long-range PCR (long PCR) covering 5,959 bp of the published sequence in vanB1 strain V583. Restriction analysis of long PCR amplicons displayed one unique vanB1 pattern and a second vanB2- and vanB3-specific pattern. The vanSB-vanYB intergenic sequences with flanking coding regions"],"journal":["Antimicrobial agents and chemotherapy"],"pagination":["1105-10"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC89118"],"repository":["biostudies-literature"],"pubmed_title":["Heterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci."],"pmcid":["PMC89118"],"pubmed_authors":["Simonsen GS","Dahl KH","Olsvik O","Sundsfjord A"],"additional_accession":[]},"is_claimable":false,"name":"Heterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci.","description":"Molecular analysis of 17 genomically unrelated clinical VanB-type vancomycin-resistant enterococcus isolates from hospital patients in Germany, Norway, Sweden, the United Kingdom and the United States revealed three subtypes of the vanB gene cluster-vanB1, vanB2, and vanB3-which was in accordance with previous subtyping of the ligase gene sequence. There was no correlation between vanB subtype and levels of vancomycin resistance. All strains studied carried a structurally conserved vanB gene cluster as shown by long-range PCR (long PCR) covering 5,959 bp of the published sequence in vanB1 strain V583. Restriction analysis of long PCR amplicons displayed one unique vanB1 pattern and a second vanB2- and vanB3-specific pattern. The vanSB-vanYB intergenic sequences with flanking coding regions","dates":{"release":"1999-01-01T00:00:00Z","publication":"1999 May","modification":"2025-04-04T13:22:33.548Z","creation":"2019-03-27T00:18:24Z"},"accession":"S-EPMC89118","cross_references":{"pubmed":["10223921"],"doi":["10.1128/AAC.43.5.1105"]}}