biostudies-literatureUnknown43(5)Dahl KHMolecular analysis of 17 genomically unrelated clinical VanB-type vancomycin-resistant enterococcus isolates from hospital patients in Germany, Norway, Sweden, the United Kingdom and the United States revealed three subtypes of the vanB gene cluster-vanB1, vanB2, and vanB3-which was in accordance with previous subtyping of the ligase gene sequence. There was no correlation between vanB subtype and levels of vancomycin resistance. All strains studied carried a structurally conserved vanB gene cluster as shown by long-range PCR (long PCR) covering 5,959 bp of the published sequence in vanB1 strain V583. Restriction analysis of long PCR amplicons displayed one unique vanB1 pattern and a second vanB2- and vanB3-specific pattern. The vanSB-vanYB intergenic sequences with flanking coding regionsAntimicrobial agents and chemotherapy1105-10https://www.ebi.ac.uk/biostudies/studies/S-EPMC89118biostudies-literatureHeterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci.PMC89118Simonsen GSDahl KHOlsvik OSundsfjord AfalseHeterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci.Molecular analysis of 17 genomically unrelated clinical VanB-type vancomycin-resistant enterococcus isolates from hospital patients in Germany, Norway, Sweden, the United Kingdom and the United States revealed three subtypes of the vanB gene cluster-vanB1, vanB2, and vanB3-which was in accordance with previous subtyping of the ligase gene sequence. There was no correlation between vanB subtype and levels of vancomycin resistance. All strains studied carried a structurally conserved vanB gene cluster as shown by long-range PCR (long PCR) covering 5,959 bp of the published sequence in vanB1 strain V583. Restriction analysis of long PCR amplicons displayed one unique vanB1 pattern and a second vanB2- and vanB3-specific pattern. The vanSB-vanYB intergenic sequences with flanking coding regions1999-01-01T00:00:00Z1999 May2025-04-04T13:22:33.548Z2019-03-27T00:18:24ZS-EPMC891181022392110.1128/AAC.43.5.1105