biostudies-literatureZhang KNational Natural Science Foundation of ChinaNational Key Research and Development Program of China609https://www.ebi.ac.uk/biostudies/studies/S-EPMC8912397biostudies-literatureUnknown11(5)<i>Brassica napus</i> (oilseed rape) is one of the most important oil crops worldwide, but its growth is seriously threatened by <i>Sclerotinia sclerotiorum</i>. The mechanism of oilseed rape response to this pathogen has rarely been studied. Here, it was identified that BnaA03.MKK5 whose expression was induced by <i>S. sclerotiorum</i> infection was involved in plant immunity. <i>BnaA03.MKK5</i> overexpression lines exhibited decreased disease symptoms compared to wild-type plants, accompanied by the increased expression of camalexin-biosynthesis-related genes, including <i>BnPAD3</i> and <i>BnCYP71A13</i>. In addition, two copies of <i>BnMPK3</i> (<i>BnA06.MPK3</i> and <i>BnC03.MPK3</i>) were induced by <i>Sclerotinia</i> incubation, and BnaA03.MKK5 interacted with BnaA06.MPK3/BnaC03.MPKPlants (Basel, Switzerland)BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i>.PMC89123972016YFD010030531376120Fu TWen JShen JMa CZhang KYi BTu JWang ZLiu FZhuo CfalseBnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i>.<i>Brassica napus</i> (oilseed rape) is one of the most important oil crops worldwide, but its growth is seriously threatened by <i>Sclerotinia sclerotiorum</i>. The mechanism of oilseed rape response to this pathogen has rarely been studied. Here, it was identified that BnaA03.MKK5 whose expression was induced by <i>S. sclerotiorum</i> infection was involved in plant immunity. <i>BnaA03.MKK5</i> overexpression lines exhibited decreased disease symptoms compared to wild-type plants, accompanied by the increased expression of camalexin-biosynthesis-related genes, including <i>BnPAD3</i> and <i>BnCYP71A13</i>. In addition, two copies of <i>BnMPK3</i> (<i>BnA06.MPK3</i> and <i>BnC03.MPK3</i>) were induced by <i>Sclerotinia</i> incubation, and BnaA03.MKK5 interacted with BnaA06.MPK3/BnaC03.MPK2022-01-01T00:00:00Z2022 Feb2026-04-08T17:35:59.882Z2025-04-04T22:09:00.14ZS-EPMC89123973527007910.3390/plants11050609