{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(1)"],"submitter":["Azuma T"],"pubmed_abstract":["Temperature sensing is a promising method of enhancing the detection sensitivity of lateral flow immunoassay (LFIA) for point-of-care testing. A temperature increase of more than 100 °C can be readily achieved by photoexcitation of reporters like gold nanoparticles (GNPs) or colored latex beads (CLBs) on LFIA strips with a laser power below 100 mW. Despite its promise, processes involved in the photothermal detection have not yet been well-characterized. Here, we provide a fundamental understanding of this thermometric assay using non-fluorescent CLBs as the reporters deposited on nitrocellulose membrane. From a measurement for the dependence of temperature rises on the number density of membrane-bound CLBs, we found a 1.3-fold (and 3.2-fold) enhancement of the light absorption by red (and"],"journal":["Scientific reports"],"pagination":["3905"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8913781"],"repository":["biostudies-literature"],"pubmed_title":["Thermometric lateral flow immunoassay with colored latex beads as reporters for COVID-19 testing."],"pmcid":["PMC8913781"],"pubmed_authors":["Wang YL","Chen OY","Hui YY","Azuma T","Chang HC"],"additional_accession":[]},"is_claimable":false,"name":"Thermometric lateral flow immunoassay with colored latex beads as reporters for COVID-19 testing.","description":"Temperature sensing is a promising method of enhancing the detection sensitivity of lateral flow immunoassay (LFIA) for point-of-care testing. A temperature increase of more than 100 °C can be readily achieved by photoexcitation of reporters like gold nanoparticles (GNPs) or colored latex beads (CLBs) on LFIA strips with a laser power below 100 mW. Despite its promise, processes involved in the photothermal detection have not yet been well-characterized. Here, we provide a fundamental understanding of this thermometric assay using non-fluorescent CLBs as the reporters deposited on nitrocellulose membrane. From a measurement for the dependence of temperature rises on the number density of membrane-bound CLBs, we found a 1.3-fold (and 3.2-fold) enhancement of the light absorption by red (and","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-05T11:07:39.701Z","creation":"2025-04-05T11:07:39.701Z"},"accession":"S-EPMC8913781","cross_references":{"pubmed":["35273286"],"doi":["10.1038/s41598-022-07963-1"]}}