biostudies-literatureUnknown9Abubakar BTertiary Education Trust Fund<i>Vernonia glaberrima</i> leaves are traditionally used to alleviate bodily pain, skin cancer, and other skin-related disorders. The purpose of the study was to investigate the acute and sub-acute toxicity of 5-methylcoumarin-4β-glucoside, a promising chemotherapeutic agent against colon cancer isolated from the leaves of <i>Vernonia glaberrima</i>. 5-methylcoumarin-4β-glucoside was isolated from the methanol leaf extract of <i>Vernonia glaberrima</i> following a previously described method. The acute toxicity study involved a two-phase 24 h observation for signs of mortality and toxicity following single oral dose administration of the isolated compound. For the sub-acute study, four groups of mice, averagely aged eight weeks, were administered graded doses of the compound (250, 500 and Toxicology reports366-372https://www.ebi.ac.uk/biostudies/studies/S-EPMC8914464biostudies-literatureEvaluation of acute and sub-acute toxicity profile of 5-methylcoumarin-4β-glucoside in mice.PMC8914464Malami IAlhassan AMUthman YAOlatubosun MOAbubakar BImam MUAdeshina KAUsman DfalseEvaluation of acute and sub-acute toxicity profile of 5-methylcoumarin-4β-glucoside in mice.<i>Vernonia glaberrima</i> leaves are traditionally used to alleviate bodily pain, skin cancer, and other skin-related disorders. The purpose of the study was to investigate the acute and sub-acute toxicity of 5-methylcoumarin-4β-glucoside, a promising chemotherapeutic agent against colon cancer isolated from the leaves of <i>Vernonia glaberrima</i>. 5-methylcoumarin-4β-glucoside was isolated from the methanol leaf extract of <i>Vernonia glaberrima</i> following a previously described method. The acute toxicity study involved a two-phase 24 h observation for signs of mortality and toxicity following single oral dose administration of the isolated compound. For the sub-acute study, four groups of mice, averagely aged eight weeks, were administered graded doses of the compound (250, 500 and 2022-01-01T00:00:00Z20222025-04-06T19:24:56.014Z2025-04-06T19:24:56.014ZS-EPMC89144643528424310.1016/j.toxrep.2022.03.013