{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song M"],"funding":["NICHD NIH HHS","NHLBI NIH HHS"],"pagination":["eabg0834"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8916722"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(10)"],"pubmed_abstract":["GATA4/5/6 transcription factors play essential, conserved roles in heart development. To understand how GATA4/5/6 modulates the mesoderm-to-cardiac fate transition, we labeled, isolated, and performed single-cell gene expression analysis on cells that express <i>gata5</i> at precardiac time points spanning zebrafish gastrulation to somitogenesis. We found that most mesendoderm-derived lineages had dynamic <i>gata5/6</i> expression. In the absence of Gata5/6, the population structure of mesendoderm-derived cells was substantially altered. In addition to the expected absence of cardiac mesoderm, we confirmed a concomitant expansion of cranial-pharyngeal mesoderm. Moreover, Gata5/6 loss led to extensive changes in chromatin accessibility near cardiac and pharyngeal genes. Functional analyses "],"journal":["Science advances"],"pubmed_title":["GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate."],"pmcid":["PMC8916722"],"funding_grant_id":["R01 HL108643","R01 HD096770"],"pubmed_authors":["Christiaen L","Song M","Leslie M","Aleksandrova A","Wilson MD","Scott IC","Yuan X","Stutt N","Racioppi C"],"additional_accession":[]},"is_claimable":false,"name":"GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate.","description":"GATA4/5/6 transcription factors play essential, conserved roles in heart development. To understand how GATA4/5/6 modulates the mesoderm-to-cardiac fate transition, we labeled, isolated, and performed single-cell gene expression analysis on cells that express <i>gata5</i> at precardiac time points spanning zebrafish gastrulation to somitogenesis. We found that most mesendoderm-derived lineages had dynamic <i>gata5/6</i> expression. In the absence of Gata5/6, the population structure of mesendoderm-derived cells was substantially altered. In addition to the expected absence of cardiac mesoderm, we confirmed a concomitant expansion of cranial-pharyngeal mesoderm. Moreover, Gata5/6 loss led to extensive changes in chromatin accessibility near cardiac and pharyngeal genes. Functional analyses ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-05T11:07:18.473Z","creation":"2025-04-05T11:07:18.473Z"},"accession":"S-EPMC8916722","cross_references":{"pubmed":["35275720"],"doi":["10.1126/sciadv.abg0834"]}}