{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang H"],"funding":["University of Michigan","NEI NIH HHS","Midwest Eye-Banks","National Institutes of Health"],"pagination":["dev199484"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8917416"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["149(2)"],"pubmed_abstract":["The mammalian retina contains a complex mixture of different types of neurons. We find that microRNA miR-216b is preferentially expressed in postmitotic retinal amacrine cells in the mouse retina, and expression of miR-216a/b and miR-217 in retina depend in part on Ptf1a, a transcription factor required for amacrine cell differentiation. Surprisingly, ectopic expression of miR-216b directed the formation of additional amacrine cells and reduced bipolar neurons in the developing retina. We identify the Foxn3 mRNA as a retinal target of miR-216b by Argonaute PAR-CLIP and reporter analysis. Inhibition of Foxn3, a transcription factor, in the postnatal developing retina by RNAi increased the formation of amacrine cells and reduced bipolar cell formation. Foxn3 disruption by CRISPR in embryonic"],"journal":["Development (Cambridge, England)"],"pubmed_title":["Regulation of retinal amacrine cell generation by miR-216b and Foxn3."],"pmcid":["PMC8917416"],"funding_grant_id":["R21 EY018707","R01EY024996","R01 EY024996","P30 EY007003","R21EY018707"],"pubmed_authors":["Zhuang P","Meng F","Dai M","Turner DL","Zhang H","Welchko RM"],"additional_accession":[]},"is_claimable":false,"name":"Regulation of retinal amacrine cell generation by miR-216b and Foxn3.","description":"The mammalian retina contains a complex mixture of different types of neurons. We find that microRNA miR-216b is preferentially expressed in postmitotic retinal amacrine cells in the mouse retina, and expression of miR-216a/b and miR-217 in retina depend in part on Ptf1a, a transcription factor required for amacrine cell differentiation. Surprisingly, ectopic expression of miR-216b directed the formation of additional amacrine cells and reduced bipolar neurons in the developing retina. We identify the Foxn3 mRNA as a retinal target of miR-216b by Argonaute PAR-CLIP and reporter analysis. Inhibition of Foxn3, a transcription factor, in the postnatal developing retina by RNAi increased the formation of amacrine cells and reduced bipolar cell formation. Foxn3 disruption by CRISPR in embryonic","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-05-09T22:39:01.892Z","creation":"2025-02-19T03:27:14.949Z"},"accession":"S-EPMC8917416","cross_references":{"pubmed":["34919141"],"doi":["10.1242/dev.199484"]}}