biostudies-literatureZhang HUniversity of MichiganNEI NIH HHSMidwest Eye-BanksNational Institutes of Healthdev199484https://www.ebi.ac.uk/biostudies/studies/S-EPMC8917416biostudies-literatureUnknown149(2)The mammalian retina contains a complex mixture of different types of neurons. We find that microRNA miR-216b is preferentially expressed in postmitotic retinal amacrine cells in the mouse retina, and expression of miR-216a/b and miR-217 in retina depend in part on Ptf1a, a transcription factor required for amacrine cell differentiation. Surprisingly, ectopic expression of miR-216b directed the formation of additional amacrine cells and reduced bipolar neurons in the developing retina. We identify the Foxn3 mRNA as a retinal target of miR-216b by Argonaute PAR-CLIP and reporter analysis. Inhibition of Foxn3, a transcription factor, in the postnatal developing retina by RNAi increased the formation of amacrine cells and reduced bipolar cell formation. Foxn3 disruption by CRISPR in embryonicDevelopment (Cambridge, England)Regulation of retinal amacrine cell generation by miR-216b and Foxn3.PMC8917416R21 EY018707R01EY024996R01 EY024996P30 EY007003R21EY018707Zhuang PMeng FDai MTurner DLZhang HWelchko RMfalseRegulation of retinal amacrine cell generation by miR-216b and Foxn3.The mammalian retina contains a complex mixture of different types of neurons. We find that microRNA miR-216b is preferentially expressed in postmitotic retinal amacrine cells in the mouse retina, and expression of miR-216a/b and miR-217 in retina depend in part on Ptf1a, a transcription factor required for amacrine cell differentiation. Surprisingly, ectopic expression of miR-216b directed the formation of additional amacrine cells and reduced bipolar neurons in the developing retina. We identify the Foxn3 mRNA as a retinal target of miR-216b by Argonaute PAR-CLIP and reporter analysis. Inhibition of Foxn3, a transcription factor, in the postnatal developing retina by RNAi increased the formation of amacrine cells and reduced bipolar cell formation. Foxn3 disruption by CRISPR in embryonic2022-01-01T00:00:00Z2022 Jan2026-05-09T22:39:01.892Z2025-02-19T03:27:14.949ZS-EPMC89174163491914110.1242/dev.199484