biostudies-literatureCai ZHainan Provincial Natural Science Foundation of ChinaNational innovation and entrepreneurship training program for college students of Hainan UniversityNational Natural Science Foundation of ChinaHainan University Scientific Research Startup Fund Projecte13079https://www.ebi.ac.uk/biostudies/studies/S-EPMC8917799biostudies-literatureUnknown10Ferns are important components of plant communities on earth, but their genomes are generally very large, with many redundant genes, making whole genome sequencing of ferns prohibitively expensive and time-consuming. This means there is a significant lack of fern reference genomes, making molecular biology research difficult. The gametophytes of ferns can survive independently, are responsible for sexual reproduction and the feeding of young sporophytes, and play an important role in the alternation of generations. For this study, we selected <i>Adiantum flabellulatum</i> as it has both ornamental and medicinal value and is also an indicator plant of acidic soil. The full-length transcriptome sequencing of its gametophytes was carried out using PacBio three-generation sequencing technologyPeerJFull-length transcriptome analysis of <i>Adiantum flabellulatum</i> gametophyte.PMC8917799No.31660229No.319MS017No.kyqd1620Luo JXie ZCai ZXu SHuang LWang ZYu XPan MfalseFull-length transcriptome analysis of <i>Adiantum flabellulatum</i> gametophyte.Ferns are important components of plant communities on earth, but their genomes are generally very large, with many redundant genes, making whole genome sequencing of ferns prohibitively expensive and time-consuming. This means there is a significant lack of fern reference genomes, making molecular biology research difficult. The gametophytes of ferns can survive independently, are responsible for sexual reproduction and the feeding of young sporophytes, and play an important role in the alternation of generations. For this study, we selected <i>Adiantum flabellulatum</i> as it has both ornamental and medicinal value and is also an indicator plant of acidic soil. The full-length transcriptome sequencing of its gametophytes was carried out using PacBio three-generation sequencing technology2022-01-01T00:00:00Z20222026-04-08T17:42:17.856Z2025-04-06T12:11:11.379ZS-EPMC89177993528734310.7717/peerj.13079