biostudies-literatureUnknown12Qi ZNatural Science Foundation of Beijing MunicipalityNational Natural Science Foundation of ChinaDespite recent evidence suggesting that nerve transfer techniques help improve clinical outcomes, the underlying manner by which collateral-regenerated nerve enters skeletal muscles to restore an organized pattern of the neuromuscular junction (NMJ) is unclear. To construct the animal models of collateral regeneration, the proximal peroneal nerve was fixed to the distal tibial nerve stump. Three months after surgery, the spatial distribution of motor endplates (MEPs) and corresponding in-muscle nerve branches in long flexor digitorum muscles were observed with tissue optical clearing combined with light-sheet microscopy in transgenic fluorescent mice. The results showed that the number of fibers in the proximal donor peroneal nerve was 415 ± 11, while regenerated nerve fibers in the distalFrontiers in physiology795623https://www.ebi.ac.uk/biostudies/studies/S-EPMC8919963biostudies-literatureStudies on the Manner of Collateral Regeneration From Nerve Stem to Motor Endplate.PMC8919963Yin XMeng DHuang CQi ZGu XLi DJin BWang SDeng JLi LfalseStudies on the Manner of Collateral Regeneration From Nerve Stem to Motor Endplate.Despite recent evidence suggesting that nerve transfer techniques help improve clinical outcomes, the underlying manner by which collateral-regenerated nerve enters skeletal muscles to restore an organized pattern of the neuromuscular junction (NMJ) is unclear. To construct the animal models of collateral regeneration, the proximal peroneal nerve was fixed to the distal tibial nerve stump. Three months after surgery, the spatial distribution of motor endplates (MEPs) and corresponding in-muscle nerve branches in long flexor digitorum muscles were observed with tissue optical clearing combined with light-sheet microscopy in transgenic fluorescent mice. The results showed that the number of fibers in the proximal donor peroneal nerve was 415 ± 11, while regenerated nerve fibers in the distal2021-01-01T00:00:00Z20212025-04-26T04:37:42.973Z2025-04-06T11:12:10.934ZS-EPMC89199633529516210.3389/fphys.2021.795623