biostudies-literatureThoming JGDeutsche ForschungsgemeinschaftEuropean Research CouncilNovo Nordisk Fonden851784https://www.ebi.ac.uk/biostudies/studies/S-EPMC8920030biostudies-literatureUnknown12Biofilm-associated bacteria exhibit profound changes in bacterial physiology. They thrive in the environment but also in the human host in protected sessile communities. Antimicrobial therapy usually fails, despite the absence of genotypic resistance, and it is commonly accepted that biofilm-grown bacteria are up to 1,000-fold more resistant than planktonic cells. We are only at the beginning to understand the reasons for biofilm recalcitrance, and systematic approaches to describe biofilm-induced tolerance phenotypes are lacking. In this study, we investigated a large and highly diverse collection of 352 clinical <i>Pseudomonas aeruginosa</i> isolates for their antimicrobial susceptibility profiles under biofilm growth conditions towards the antibiotics ciprofloxacin, tobramycin, and coliFrontiers in cellular and infection microbiology<i>Pseudomonas aeruginosa</i> Is More Tolerant Under Biofilm Than Under Planktonic Growth Conditions: A Multi-Isolate Survey.PMC8920030NNF 18OC0033946724290SPP 1879COMBAT 724290Thoming JGHaussler Sfalse<i>Pseudomonas aeruginosa</i> Is More Tolerant Under Biofilm Than Under Planktonic Growth Conditions: A Multi-Isolate Survey.Biofilm-associated bacteria exhibit profound changes in bacterial physiology. They thrive in the environment but also in the human host in protected sessile communities. Antimicrobial therapy usually fails, despite the absence of genotypic resistance, and it is commonly accepted that biofilm-grown bacteria are up to 1,000-fold more resistant than planktonic cells. We are only at the beginning to understand the reasons for biofilm recalcitrance, and systematic approaches to describe biofilm-induced tolerance phenotypes are lacking. In this study, we investigated a large and highly diverse collection of 352 clinical <i>Pseudomonas aeruginosa</i> isolates for their antimicrobial susceptibility profiles under biofilm growth conditions towards the antibiotics ciprofloxacin, tobramycin, and coli2022-01-01T00:00:00Z20222025-04-04T07:44:30.151Z2025-04-04T07:44:30.151ZS-EPMC89200303529575510.3389/fcimb.2022.851784