{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jevtic P"],"funding":["NASA","National Aeronautics and Space Administration","National Institutes of Health","National Institute of General Medical Sciences","NIGMS NIH HHS","NIH HHS","National Science Foundation"],"pagination":["jeb243662"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8920033"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["225(4)"],"pubmed_abstract":["Cryoprotection is of interest in many fields of research, necessitating a greater understanding of different cryoprotective agents. Antifreeze proteins have been identified that have the ability to confer cryoprotection in certain organisms. Antifreeze proteins are an evolutionary adaptation that contributes to the freeze resistance of certain fish, insects, bacteria and plants. These proteins adsorb to an ice crystal's surface and restrict its growth within a certain temperature range. We investigated the ability of an antifreeze protein from the desert beetle Anatolica polita, ApAFP752, to confer cryoprotection in the frog Xenopus laevis. Xenopus laevis eggs and embryos microinjected with ApAFP752 exhibited reduced damage and increased survival after a freeze-thaw cycle in a concentratio"],"journal":["The Journal of experimental biology"],"pubmed_title":["An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos."],"pmcid":["PMC8920033"],"funding_grant_id":["CHE-1413696","NNX10A095H","P20GM103432","80NSSC18M0034","R35 GM134885","DGE-0948027","GM135903","R35GM134885","P20 GM103432","CHE-1740399","R15 GM135903"],"pubmed_authors":["Jovic K","Baures PW","Watkins SE","Lehner IB","Levy DL","Elliott KW","Tsavalas JG","Varga K","Sreter JA","Jevtic P"],"additional_accession":[]},"is_claimable":false,"name":"An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos.","description":"Cryoprotection is of interest in many fields of research, necessitating a greater understanding of different cryoprotective agents. Antifreeze proteins have been identified that have the ability to confer cryoprotection in certain organisms. Antifreeze proteins are an evolutionary adaptation that contributes to the freeze resistance of certain fish, insects, bacteria and plants. These proteins adsorb to an ice crystal's surface and restrict its growth within a certain temperature range. We investigated the ability of an antifreeze protein from the desert beetle Anatolica polita, ApAFP752, to confer cryoprotection in the frog Xenopus laevis. Xenopus laevis eggs and embryos microinjected with ApAFP752 exhibited reduced damage and increased survival after a freeze-thaw cycle in a concentratio","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2026-06-04T04:57:07.249Z","creation":"2025-04-21T14:20:31.843Z"},"accession":"S-EPMC8920033","cross_references":{"pubmed":["35014670"],"doi":["10.1242/jeb.243662"]}}