biostudies-literatureTraver MSNational Institutes of HealthWelch FoundationNIGMS NIH HHSNIH HHSNational Science Foundation838923https://www.ebi.ac.uk/biostudies/studies/S-EPMC8922245biostudies-literatureUnknown10Peroxisomes are eukaryotic organelles that sequester critical oxidative reactions and process the resulting reactive oxygen species into less toxic byproducts. Peroxisome function and formation are coordinated by peroxins (PEX proteins) that guide peroxisome biogenesis and division and shuttle proteins into the lumen and membrane of the organelle. Despite the importance of peroxins in plant metabolism and development, no plant peroxin structures have been reported. Here we report the X-ray crystal structure of the PEX4-PEX22 peroxin complex from the reference plant <i>Arabidopsis thaliana</i>. PEX4 is a ubiquitin-conjugating enzyme (UBC) that ubiquitinates proteins associated with the peroxisomal membrane, and PEX22 is a peroxisomal membrane protein that anchors PEX4 to the peroxisome and Frontiers in cell and developmental biologyThe Structure of the <i>Arabidopsis</i> PEX4-PEX22 Peroxin Complex-Insights Into Ubiquitination at the Peroxisomal Membrane.PMC8922245R01 GM129325R35 GM130338P30 GM138396S10 OD012289Traver MSOlmos JLPhillips GNBradford SEWright ZJXu WMiller MDBartel BfalseThe Structure of the <i>Arabidopsis</i> PEX4-PEX22 Peroxin Complex-Insights Into Ubiquitination at the Peroxisomal Membrane.Peroxisomes are eukaryotic organelles that sequester critical oxidative reactions and process the resulting reactive oxygen species into less toxic byproducts. Peroxisome function and formation are coordinated by peroxins (PEX proteins) that guide peroxisome biogenesis and division and shuttle proteins into the lumen and membrane of the organelle. Despite the importance of peroxins in plant metabolism and development, no plant peroxin structures have been reported. Here we report the X-ray crystal structure of the PEX4-PEX22 peroxin complex from the reference plant <i>Arabidopsis thaliana</i>. PEX4 is a ubiquitin-conjugating enzyme (UBC) that ubiquitinates proteins associated with the peroxisomal membrane, and PEX22 is a peroxisomal membrane protein that anchors PEX4 to the peroxisome and 2022-01-01T00:00:00Z20222026-04-08T17:32:44.449Z2025-04-04T22:08:56.286ZS-EPMC89222453530042510.3389/fcell.2022.838923