{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li N"],"funding":["Fundamental Research Funds for the Central Universities","National First-class Discipline Program of Light Industry Technology and Engineering","National Natural Science Foundation of China","Jiangsu Postdoctoral Research Funding Program","National Key Research and Development Program of China"],"pagination":["27"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8922893"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Background
O-Acetyl-L-homoserine (OAH) is an important potential platform chemical. However, low levels of production of OAH are greatly limiting its industrial application. Furthermore, as a common and safe amino acid-producing strain, Corynebacterium glutamicum has not yet achieved efficient production of OAH.Results
First, exogenous L-homoserine acetyltransferase was introduced into an L-homoserine-producing strain, resulting in the accumulation of 0.98 g/L of OAH. Second, by comparing different acetyl-CoA biosynthesis pathways and adding several feedstocks (acetate, citrate, and pantothenate), the OAH titer increased 2.3-fold to 3.2 g/L. Then, the OAH titer further increased by 62.5% when the expression of L-homoserine dehydrogenase and L-homoserine acetyltransferase was strengthened via strong promoters. Finally, the engineered strain produced 17.4 g/L of OAH in 96 h with acetate as the supplementary feedstock in a 5-L bioreactor.Conclusions
This is the first report on the efficient production of OAH with C. glutamicum as the chassis, which would provide a good foundation for industrial production of OAH."],"journal":["Biotechnology for biofuels and bioproducts"],"pubmed_title":["O-Acetyl-L-homoserine production enhanced by pathway strengthening and acetate supplementation in Corynebacterium glutamicum."],"pmcid":["PMC8922893"],"funding_grant_id":["2021K171B","22108098","JUSRP121118","LITE2018-08","2019YFA0904800"],"pubmed_authors":["Xu S","Zeng W","Li N","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"O-Acetyl-L-homoserine production enhanced by pathway strengthening and acetate supplementation in Corynebacterium glutamicum.","description":"Background
O-Acetyl-L-homoserine (OAH) is an important potential platform chemical. However, low levels of production of OAH are greatly limiting its industrial application. Furthermore, as a common and safe amino acid-producing strain, Corynebacterium glutamicum has not yet achieved efficient production of OAH.Results
First, exogenous L-homoserine acetyltransferase was introduced into an L-homoserine-producing strain, resulting in the accumulation of 0.98 g/L of OAH. Second, by comparing different acetyl-CoA biosynthesis pathways and adding several feedstocks (acetate, citrate, and pantothenate), the OAH titer increased 2.3-fold to 3.2 g/L. Then, the OAH titer further increased by 62.5% when the expression of L-homoserine dehydrogenase and L-homoserine acetyltransferase was strengthened via strong promoters. Finally, the engineered strain produced 17.4 g/L of OAH in 96 h with acetate as the supplementary feedstock in a 5-L bioreactor.Conclusions
This is the first report on the efficient production of OAH with C. glutamicum as the chassis, which would provide a good foundation for industrial production of OAH.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-26T19:11:05.185Z","creation":"2025-04-06T16:00:37.669Z"},"accession":"S-EPMC8922893","cross_references":{"pubmed":["35287716"],"doi":["10.1186/s13068-022-02114-0"]}}