biostudies-literatureWaxman SNEI NIH HHSNICHD NIH HHS108916https://www.ebi.ac.uk/biostudies/studies/S-EPMC8923914biostudies-literatureUnknown215Our goal was to analyze the spatial interrelation between vascular and collagen networks in the lamina cribrosa (LC). Specifically, we quantified the percentages of collagen beams with/without vessels and of vessels inside/outside of collagen beams. To do this, the vasculature of six normal monkey eyes was labeled by perfusion post-mortem. After enucleation, coronal cryosections through the LC were imaged using fluorescence and polarized light microscopy to visualize the blood vessels and collagen beams, respectively. The images were registered to form 3D volumes. Beams and vessels were segmented, and their spatial interrelationship was quantified in 3D. We found that 22% of the beams contained a vessel (range 14%-32%), and 21% of vessels were outside beams (13%-36%). Stated differently, 7Experimental eye researchLamina cribrosa vessel and collagen beam networks are distinct.PMC8923914T32 EY017271P30 EY008098R01 EY023966R01 EY028662R01 HD083383R01 HD045590R01 EY031708Hua YBrazile BLGogola ALLee PYLam PSigal IAWaxman SYang BVoorhees APJakobs TCRizzo JFfalseLamina cribrosa vessel and collagen beam networks are distinct.Our goal was to analyze the spatial interrelation between vascular and collagen networks in the lamina cribrosa (LC). Specifically, we quantified the percentages of collagen beams with/without vessels and of vessels inside/outside of collagen beams. To do this, the vasculature of six normal monkey eyes was labeled by perfusion post-mortem. After enucleation, coronal cryosections through the LC were imaged using fluorescence and polarized light microscopy to visualize the blood vessels and collagen beams, respectively. The images were registered to form 3D volumes. Beams and vessels were segmented, and their spatial interrelationship was quantified in 3D. We found that 22% of the beams contained a vessel (range 14%-32%), and 21% of vessels were outside beams (13%-36%). Stated differently, 72022-01-01T00:00:00Z2022 Feb2025-04-05T09:06:07.678Z2025-04-05T09:06:07.678ZS-EPMC89239143497320410.1016/j.exer.2021.108916