{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14"],"submitter":["Wang T"],"pubmed_abstract":["Tissue engineering provides a new approach for the treatment of osteochondral defects. However, the lack of an ideal double-layer scaffold with osteochondral-biomimetic microenvironment and interface similar to native articular tissue greatly limits clinical translation. Our current study developed a double-layer acellular osteochondral matrix (AOM) scaffold with natural osteochondral-biomimetic microenvironment and interface by integrating ultraviolet (UV) laser and decellularization techniques. The laser parameters were optimized to achieve a proper pore depth close to the osteochondral interface, which guaranteed complete decellularization, sufficient space for cell loading, and relative independence of the chondrogenic and osteogenic microenvironments. Gelatin-methacryloyl (GelMA) hydr"],"journal":["Materials today. Bio"],"pagination":["100234"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8924418"],"repository":["biostudies-literature"],"pubmed_title":["Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface."],"pmcid":["PMC8924418"],"pubmed_authors":["Hua Y","Tang J","Liu Y","Bai B","Wang T","Cao Y","Chen F","Zhou G","Wang Y","Xu W","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface.","description":"Tissue engineering provides a new approach for the treatment of osteochondral defects. However, the lack of an ideal double-layer scaffold with osteochondral-biomimetic microenvironment and interface similar to native articular tissue greatly limits clinical translation. Our current study developed a double-layer acellular osteochondral matrix (AOM) scaffold with natural osteochondral-biomimetic microenvironment and interface by integrating ultraviolet (UV) laser and decellularization techniques. The laser parameters were optimized to achieve a proper pore depth close to the osteochondral interface, which guaranteed complete decellularization, sufficient space for cell loading, and relative independence of the chondrogenic and osteogenic microenvironments. Gelatin-methacryloyl (GelMA) hydr","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-19T00:51:18.283Z","creation":"2025-04-07T11:42:37.949Z"},"accession":"S-EPMC8924418","cross_references":{"pubmed":["35308043"],"doi":["10.1016/j.mtbio.2022.100234"]}}