biostudies-literatureSun CHealth and Family Planning Commission of Wuhan Municipality3421600https://www.ebi.ac.uk/biostudies/studies/S-EPMC8924609biostudies-literatureUnknown2022Osteosarcoma is the most common primary malignant bone tumor in children and adolescents with poor prognosis. MicroRNA-181a-5p (miR-181a-5p) is involved in the progression of various tumors; however, its role and underlying mechanism in osteosarcoma remains unclear. In this study, we found that miR-181a-5p was upregulated in human osteosarcoma cells and tissues. miR-181a-5p mimic significantly promoted, while miR-181a-5p inhibitor blocked the proliferation, colony formation, migration, invasion, and cell cycle progression of osteosarcoma cells. Mechanistically, miR-181a-5p bound to the 3'-untranslational region of phosphatase and tensin homolog (PTEN) and reduced its protein expression, thereby activating protein kinase B (PKB/AKT) pathway. Either PTEN overexpression or AKT inhibition notaAnalytical cellular pathology (Amsterdam)MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway.PMC8924609WX19Q15Qian WAi FGuo JXiao LChen ZGuo XSun CYu ZHChen CfalseMicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway.Osteosarcoma is the most common primary malignant bone tumor in children and adolescents with poor prognosis. MicroRNA-181a-5p (miR-181a-5p) is involved in the progression of various tumors; however, its role and underlying mechanism in osteosarcoma remains unclear. In this study, we found that miR-181a-5p was upregulated in human osteosarcoma cells and tissues. miR-181a-5p mimic significantly promoted, while miR-181a-5p inhibitor blocked the proliferation, colony formation, migration, invasion, and cell cycle progression of osteosarcoma cells. Mechanistically, miR-181a-5p bound to the 3'-untranslational region of phosphatase and tensin homolog (PTEN) and reduced its protein expression, thereby activating protein kinase B (PKB/AKT) pathway. Either PTEN overexpression or AKT inhibition nota2022-01-01T00:00:00Z20222025-04-26T03:53:52.028Z2025-04-06T10:57:21.203ZS-EPMC89246093531093310.1155/2022/3421600