{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hinkle ER"],"funding":["NHLBI NIH HHS","NIAMS NIH HHS","NIGMS NIH HHS"],"pagination":["523-540"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8925968"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(4)"],"pubmed_abstract":["Alternative splicing transitions occur during organ development, and, in numerous diseases, splicing programs revert to fetal isoform expression. We previously found that extensive splicing changes occur during postnatal mouse heart development in genes encoding proteins involved in vesicle-mediated trafficking. However, the regulatory mechanisms of this splicing-trafficking network are unknown. Here, we found that membrane trafficking genes are alternatively spliced in a tissue-specific manner, with striated muscles exhibiting the highest levels of alternative exon inclusion. Treatment of differentiated muscle cells with chromatin-modifying drugs altered exon inclusion in muscle cells. Examination of several RNA-binding proteins revealed that the poly-pyrimidine tract binding protein 1 (P"],"journal":["RNA (New York, N.Y.)"],"pubmed_title":["Alternative splicing regulation of membrane trafficking genes during myogenesis."],"pmcid":["PMC8925968"],"funding_grant_id":["T32 GM008570","R25 GM055336","T32 GM135095","F32 HL149147","R01 GM130866","T32 GM007092","F31 AR077381","T32 GM119999"],"pubmed_authors":["Torres EV","Jackson M","Lee EY","Tsai YH","Hinkle ER","Dominguez D","Giudice J","Black AJ","Harris SE","Guzman BB","Gentile GM","Parker J","Blue RE","Wiedner HJ"],"additional_accession":[]},"is_claimable":false,"name":"Alternative splicing regulation of membrane trafficking genes during myogenesis.","description":"Alternative splicing transitions occur during organ development, and, in numerous diseases, splicing programs revert to fetal isoform expression. We previously found that extensive splicing changes occur during postnatal mouse heart development in genes encoding proteins involved in vesicle-mediated trafficking. However, the regulatory mechanisms of this splicing-trafficking network are unknown. Here, we found that membrane trafficking genes are alternatively spliced in a tissue-specific manner, with striated muscles exhibiting the highest levels of alternative exon inclusion. Treatment of differentiated muscle cells with chromatin-modifying drugs altered exon inclusion in muscle cells. Examination of several RNA-binding proteins revealed that the poly-pyrimidine tract binding protein 1 (P","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-19T13:29:12.308Z","creation":"2025-04-19T13:29:12.308Z"},"accession":"S-EPMC8925968","cross_references":{"pubmed":["35082143"],"doi":["10.1261/rna.078993.121"]}}