biostudies-literatureZhang YNatural Sciences and Engineering Research Council of Canada3233-3243https://www.ebi.ac.uk/biostudies/studies/S-EPMC8926288biostudies-literatureUnknown13(11)Mass cytometry is an emerging powerful bioanalytical technique for high-dimensional single-cell analysis. In this technique, cells are stained with metal-isotope-tagged antibodies and are analyzed by an inductively coupled plasma time-of-flight mass spectrometer. While there are more than 100 stable isotopes available in the <i>m</i>/<i>z</i> 75 to 209 detection range of the instrument, only about 50 parameters can be measured per cell because current reagents are metal-chelating polymers with pendant aminocarboxylate chelators that only bind hard metal ions such as the rare earths and Bi³⁺. Here we describe the synthesis and characterization of a new type of metal-chelating polymer with pendant dipicolylamine chelators suited to binding intermediate to soft metals such as rheniChemical sciencePolymeric dipicolylamine based mass tags for mass cytometry.PMC8926288CRDPJ 484832 – 15Zhang YMajonis DLiu PWinnik MAfalsePolymeric dipicolylamine based mass tags for mass cytometry.Mass cytometry is an emerging powerful bioanalytical technique for high-dimensional single-cell analysis. In this technique, cells are stained with metal-isotope-tagged antibodies and are analyzed by an inductively coupled plasma time-of-flight mass spectrometer. While there are more than 100 stable isotopes available in the <i>m</i>/<i>z</i> 75 to 209 detection range of the instrument, only about 50 parameters can be measured per cell because current reagents are metal-chelating polymers with pendant aminocarboxylate chelators that only bind hard metal ions such as the rare earths and Bi³⁺. Here we describe the synthesis and characterization of a new type of metal-chelating polymer with pendant dipicolylamine chelators suited to binding intermediate to soft metals such as rheni2022-01-01T00:00:00Z2022 Mar2026-05-09T17:42:05.184Z2024-11-10T08:50:04.892ZS-EPMC89262883541486810.1039/d2sc00595f