biostudies-literatureMatsumoto CCore Research for Evolutional Science and TechnologyFusion Oriented REsearch for disruptive Science and TechnologyJapan Society for the Promotion of Science4511https://www.ebi.ac.uk/biostudies/studies/S-EPMC8927375biostudies-literatureUnknown12(1)We report a spray-drying method to fabricate silver nanoparticle (AgNP) aggregates for application in surface-enhanced Raman spectroscopy (SERS). A custom-built system was used to fabricate AgNP aggregates of four sizes, 48, 86, 151, and 218 nm, from drying droplets containing AgNPs atomized from an AgNP suspension. Sample solutions of Rhodamine B (RhB) at 10^-6, 10^-8, and 10^-10 M concentrations were dropped onto the AgNP aggregates as probe molecules to examine the enhancement of the Raman signals of the RhB. The ordering of the analytical enhancement factors (AEFs) by aggregate size at a 10^-6 M RhB was 86 nm > 218 nm > 151 nm > 48 nm. When RhB concentrations are below 10^-8 M, the 86 and 151 nm AgNP aggregates show clear RhB peaks. TheScientific reportsDevelopment of spray-drying-based surface-enhanced Raman spectroscopy.PMC8927375JPMJCR18H4JPMJFR206V21H01687Matsumoto CGen MSeto TMatsuki AfalseDevelopment of spray-drying-based surface-enhanced Raman spectroscopy.We report a spray-drying method to fabricate silver nanoparticle (AgNP) aggregates for application in surface-enhanced Raman spectroscopy (SERS). A custom-built system was used to fabricate AgNP aggregates of four sizes, 48, 86, 151, and 218 nm, from drying droplets containing AgNPs atomized from an AgNP suspension. Sample solutions of Rhodamine B (RhB) at 10^-6, 10^-8, and 10^-10 M concentrations were dropped onto the AgNP aggregates as probe molecules to examine the enhancement of the Raman signals of the RhB. The ordering of the analytical enhancement factors (AEFs) by aggregate size at a 10^-6 M RhB was 86 nm > 218 nm > 151 nm > 48 nm. When RhB concentrations are below 10^-8 M, the 86 and 151 nm AgNP aggregates show clear RhB peaks. The2022-01-01T00:00:00Z2022 Mar2025-04-05T15:58:13.745Z2025-04-05T15:58:13.745ZS-EPMC89273753529677510.1038/s41598-022-08598-y