{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang D"],"funding":["National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["846321"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8927680"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["The multicopy suppressor of IRA (MSI) is a subfamily of WD40 repeat proteins, which is widely involved in plant growth and development. In order to explore the function of <i>MdMSI</i> members in abiotic stress, we identified eight <i>MSI</i> gene family members from the <i>Malus</i> × <i>domestica</i> reference genome. They were distributed on six chromosomes, and they had similar secondary and tertiary structures. We found a variety of regulatory elements in response to hormones and abiotic stress in <i>MdMSI</i> promoters. Through qRT-PCR analysis, it was revealed that <i>MdMSIs</i> were expressed in all tissues, especially in roots. The analysis results also revealed that the expression of <i>MdMSIs</i> was induced in varying degrees under salt, drought stress, and ABA treatments. Furthermore, we obtained the overexpression of <i>MdMSI1-1</i> transgenic apple calli and <i>Arabidopsis</i>. The overexpression of <i>MdMSI1-1</i> in calli and <i>Arabidopsis</i> played a negative regulatory role in salt stress response. Our work laid a foundation for further verifying the function of <i>MSI</i> genes under abiotic stress in apples."],"journal":["Frontiers in genetics"],"pubmed_title":["Genome-wide Identification, Expression, and Functional Analysis of <i>MdMSI</i> Genes in Apples (<i>Malus domestica</i> Borkh.)."],"pmcid":["PMC8927680"],"funding_grant_id":["31772288 32172538","2018YFD1000100"],"pubmed_authors":["Yang K","Wang D","Cui J","You C","Wang X","Zhang C","Liu D"],"additional_accession":[]},"is_claimable":false,"name":"Genome-wide Identification, Expression, and Functional Analysis of <i>MdMSI</i> Genes in Apples (<i>Malus domestica</i> Borkh.).","description":"The multicopy suppressor of IRA (MSI) is a subfamily of WD40 repeat proteins, which is widely involved in plant growth and development. In order to explore the function of <i>MdMSI</i> members in abiotic stress, we identified eight <i>MSI</i> gene family members from the <i>Malus</i> × <i>domestica</i> reference genome. They were distributed on six chromosomes, and they had similar secondary and tertiary structures. We found a variety of regulatory elements in response to hormones and abiotic stress in <i>MdMSI</i> promoters. Through qRT-PCR analysis, it was revealed that <i>MdMSIs</i> were expressed in all tissues, especially in roots. The analysis results also revealed that the expression of <i>MdMSIs</i> was induced in varying degrees under salt, drought stress, and ABA treatments. Furthermore, we obtained the overexpression of <i>MdMSI1-1</i> transgenic apple calli and <i>Arabidopsis</i>. The overexpression of <i>MdMSI1-1</i> in calli and <i>Arabidopsis</i> played a negative regulatory role in salt stress response. Our work laid a foundation for further verifying the function of <i>MSI</i> genes under abiotic stress in apples.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-19T12:52:24.092Z","creation":"2025-04-19T12:52:24.092Z"},"accession":"S-EPMC8927680","cross_references":{"pubmed":["35309144"],"doi":["10.3389/fgene.2022.846321"]}}