biostudies-literatureWang DNational Natural Science Foundation of ChinaNational Key Research and Development Program of China846321https://www.ebi.ac.uk/biostudies/studies/S-EPMC8927680biostudies-literatureUnknown13The multicopy suppressor of IRA (MSI) is a subfamily of WD40 repeat proteins, which is widely involved in plant growth and development. In order to explore the function of <i>MdMSI</i> members in abiotic stress, we identified eight <i>MSI</i> gene family members from the <i>Malus</i> × <i>domestica</i> reference genome. They were distributed on six chromosomes, and they had similar secondary and tertiary structures. We found a variety of regulatory elements in response to hormones and abiotic stress in <i>MdMSI</i> promoters. Through qRT-PCR analysis, it was revealed that <i>MdMSIs</i> were expressed in all tissues, especially in roots. The analysis results also revealed that the expression of <i>MdMSIs</i> was induced in varying degrees under salt, drought stress, and ABA treatments. Furthermore, we obtained the overexpression of <i>MdMSI1-1</i> transgenic apple calli and <i>Arabidopsis</i>. The overexpression of <i>MdMSI1-1</i> in calli and <i>Arabidopsis</i> played a negative regulatory role in salt stress response. Our work laid a foundation for further verifying the function of <i>MSI</i> genes under abiotic stress in apples.Frontiers in geneticsGenome-wide Identification, Expression, and Functional Analysis of <i>MdMSI</i> Genes in Apples (<i>Malus domestica</i> Borkh.).PMC892768031772288 321725382018YFD1000100Yang KWang DCui JYou CWang XZhang CLiu DfalseGenome-wide Identification, Expression, and Functional Analysis of <i>MdMSI</i> Genes in Apples (<i>Malus domestica</i> Borkh.).The multicopy suppressor of IRA (MSI) is a subfamily of WD40 repeat proteins, which is widely involved in plant growth and development. In order to explore the function of <i>MdMSI</i> members in abiotic stress, we identified eight <i>MSI</i> gene family members from the <i>Malus</i> × <i>domestica</i> reference genome. They were distributed on six chromosomes, and they had similar secondary and tertiary structures. We found a variety of regulatory elements in response to hormones and abiotic stress in <i>MdMSI</i> promoters. Through qRT-PCR analysis, it was revealed that <i>MdMSIs</i> were expressed in all tissues, especially in roots. The analysis results also revealed that the expression of <i>MdMSIs</i> was induced in varying degrees under salt, drought stress, and ABA treatments. Furthermore, we obtained the overexpression of <i>MdMSI1-1</i> transgenic apple calli and <i>Arabidopsis</i>. The overexpression of <i>MdMSI1-1</i> in calli and <i>Arabidopsis</i> played a negative regulatory role in salt stress response. Our work laid a foundation for further verifying the function of <i>MSI</i> genes under abiotic stress in apples.2022-01-01T00:00:00Z20222025-04-19T12:52:24.092Z2025-04-19T12:52:24.092ZS-EPMC89276803530914410.3389/fgene.2022.846321