biostudies-literaturede Haan NDanmarks GrundforskningsfondEuropean Research CouncilHorizon 2020 Framework ProgrammeFriis FoundationA.P. M?ller og Hustru Chastine Mc-Kinney M?llers Fond til almene FormaalLundbeck FoundationMichelsen Foundation4343-4351https://www.ebi.ac.uk/biostudies/studies/S-EPMC8928149biostudies-literatureUnknown94(10)<i>O</i>-Glycosylation is an omnipresent modification of the human proteome affecting many cellular functions, including protein cleavage, protein folding, and cellular signaling, interactions, and trafficking. The functions are governed by differentially regulated <i>O</i>-glycan types and terminal structures. It is therefore essential to develop analytical methods that facilitate the annotation of <i>O</i>-glycans in biological material. While various successful strategies for the in-depth profiling of released <i>O</i>-glycans have been reported, these methods are often limitedly accessible to the nonspecialist or challenged by the high abundance of <i>O</i>-glycan structural isomers. Here, we developed a high-throughput sample preparation approach for the nonreductive release and charaAnalytical chemistryIn-Depth Profiling of <i>O</i>-Glycan Isomers in Human Cells Using C18 Nanoliquid Chromatography-Mass Spectrometry and Glycogenomics.PMC8928149313-2019-869R313-2019-869772735DNRF107Larsen ISBWandall HHKoed Moller Aasted MDabelsteen Sde Haan NVakhrushev SYMarinova INNarimatsu YfalseIn-Depth Profiling of <i>O</i>-Glycan Isomers in Human Cells Using C18 Nanoliquid Chromatography-Mass Spectrometry and Glycogenomics.<i>O</i>-Glycosylation is an omnipresent modification of the human proteome affecting many cellular functions, including protein cleavage, protein folding, and cellular signaling, interactions, and trafficking. The functions are governed by differentially regulated <i>O</i>-glycan types and terminal structures. It is therefore essential to develop analytical methods that facilitate the annotation of <i>O</i>-glycans in biological material. While various successful strategies for the in-depth profiling of released <i>O</i>-glycans have been reported, these methods are often limitedly accessible to the nonspecialist or challenged by the high abundance of <i>O</i>-glycan structural isomers. Here, we developed a high-throughput sample preparation approach for the nonreductive release and chara2022-01-01T00:00:00Z2022 Mar2025-04-26T03:57:15.123Z2025-04-06T10:58:26.536ZS-EPMC89281493524504010.1021/acs.analchem.1c05068