biostudies-literatureStromme OLiaison Committee between the central Norway Regional Health Authority (RHA) and the Norwegian University of Science and technology (NTNU)Liaison Committee between the central Norway Regional Health Authority (RHA) and the Norwegian University of Science and technology (NTNU) (SO)Olav Raagholt og Gerd Meidel Raagholts stiftelseThe Joint Research Committee between St. Olav's Hospital and the Faculty of Medicine and Health Sciences, NTNU (FFU)Kreftfondet (Cancer Research Fund), St. Olav's Hospital286-300https://www.ebi.ac.uk/biostudies/studies/S-EPMC8929081biostudies-literatureUnknown43(1)Bladder cancer (BC) is currently diagnosed and monitored by cystoscopy, a costly and invasive procedure. Potential biomarkers in urine, blood, and, more recently, extracellular vesicles (EVs), have been explored as non-invasive alternatives for diagnosis and surveillance of BC. EVs are nanovesicles secreted by most cell types containing diverse molecular cargo, including different types of small RNAs, such as microRNA (miRNA). In this study, we performed next-generation sequencing of EV-contained miRNA isolated from urine and serum of 41 patients with non-muscle invasive BC (27 stage Ta, 14 stage T1) and 15 non-cancer patients (NCP) with benign cystoscopy findings. MiRNA sequencing was also performed on serum supernatant samples for T1 patients. To identify potential BC-specific biomarkersCurrent issues in molecular biologyDifferentially Expressed Extracellular Vesicle-Contained microRNAs before and after Transurethral Resection of Bladder Tumors.PMC89290812019/38882NA2016/43/162017/22110Otterlei MLindholm HTBrede GStromme OArum CJHeck KAfalseDifferentially Expressed Extracellular Vesicle-Contained microRNAs before and after Transurethral Resection of Bladder Tumors.Bladder cancer (BC) is currently diagnosed and monitored by cystoscopy, a costly and invasive procedure. Potential biomarkers in urine, blood, and, more recently, extracellular vesicles (EVs), have been explored as non-invasive alternatives for diagnosis and surveillance of BC. EVs are nanovesicles secreted by most cell types containing diverse molecular cargo, including different types of small RNAs, such as microRNA (miRNA). In this study, we performed next-generation sequencing of EV-contained miRNA isolated from urine and serum of 41 patients with non-muscle invasive BC (27 stage Ta, 14 stage T1) and 15 non-cancer patients (NCP) with benign cystoscopy findings. MiRNA sequencing was also performed on serum supernatant samples for T1 patients. To identify potential BC-specific biomarkers2021-01-01T00:00:00Z2021 Jun2025-04-04T09:40:53.476Z2025-04-04T09:40:53.476ZS-EPMC89290813419976610.3390/cimb43010024