biostudies-literatureUnknown44(2)Wu YSheep testes undergo a dramatic rate of development with structural changes during pre-sexual maturity, including the proliferation and maturation of somatic niche cells and the initiation of spermatogenesis. To explore this complex process, 12,843 testicular cells from three males at pre-sexual maturity (three-month-old) were sequenced using the 10× Genomics Chromium^TM single-cell RNA-seq (scRNA-seq) technology. Nine testicular somatic cell types (Sertoli cells, myoid cells, monocytes, macrophages, Leydig cells, dendritic cells, endothelial cells, smooth muscle cells, and leukocytes) and an unknown cell cluster were observed. In particular, five male germ cell types (including two types of undifferentiated spermatogonia (A_pale and A_dark), primary spermatocCurrent issues in molecular biology483-497https://www.ebi.ac.uk/biostudies/studies/S-EPMC8929108biostudies-literatureThe Transcriptional Cell Atlas of Testis Development in Sheep at Pre-Sexual Maturity.PMC8929108Qiao GNiu CLiu JLi JHan MLu ZGuo TYang BSun WYuan CHe XHan JWu YYue YZhu SZhao HfalseThe Transcriptional Cell Atlas of Testis Development in Sheep at Pre-Sexual Maturity.Sheep testes undergo a dramatic rate of development with structural changes during pre-sexual maturity, including the proliferation and maturation of somatic niche cells and the initiation of spermatogenesis. To explore this complex process, 12,843 testicular cells from three males at pre-sexual maturity (three-month-old) were sequenced using the 10× Genomics Chromium^TM single-cell RNA-seq (scRNA-seq) technology. Nine testicular somatic cell types (Sertoli cells, myoid cells, monocytes, macrophages, Leydig cells, dendritic cells, endothelial cells, smooth muscle cells, and leukocytes) and an unknown cell cluster were observed. In particular, five male germ cell types (including two types of undifferentiated spermatogonia (A_pale and A_dark), primary spermatoc2022-01-01T00:00:00Z2022 Jan2025-04-04T09:40:26.765Z2025-04-04T09:40:26.765ZS-EPMC89291083572331910.3390/cimb44020033