biostudies-literatureAghanoori MRResearch Manitoba Postdoctoral FellowshipUniversity of Manitoba Graduate FellowshipBank of Montreal (BMO) Financial GroupCanadian Institute of Health Research193https://www.ebi.ac.uk/biostudies/studies/S-EPMC8930798biostudies-literatureUnknown79(4)Aberrant insulin-like growth factor 1 (IGF-1) signaling has been proposed as a contributing factor to the development of neurodegenerative disorders including diabetic neuropathy, and delivery of exogenous IGF-1 has been explored as a treatment for Alzheimer's disease and amyotrophic lateral sclerosis. However, the role of autocrine/paracrine IGF-1 in neuroprotection has not been well established. We therefore used in vitro cell culture systems and animal models of diabetic neuropathy to characterize endogenous IGF-1 in sensory neurons and determine the factors regulating IGF-1 expression and/or affecting neuronal health. Single-cell RNA sequencing (scRNA-Seq) and in situ hybridization analyses revealed high expression of endogenous IGF-1 in non-peptidergic neurons and satellite glial cellCellular and molecular life sciences : CMLSCEBPβ regulation of endogenous IGF-1 in adult sensory neurons can be mobilized to overcome diabetes-induced deficits in bioenergetics and axonal outgrowth.PMC8930798MOP-130282Lee SGauvin EAgarwal PSmith DRJones MJAghanoori MRBonomo RMiller DWFernyhough PHai YDolinsky VWCzubryt MPMansuy-Aubert VNagalingam RSJolivalt CGYathindranath VCalcutt NAfalseCEBPβ regulation of endogenous IGF-1 in adult sensory neurons can be mobilized to overcome diabetes-induced deficits in bioenergetics and axonal outgrowth.Aberrant insulin-like growth factor 1 (IGF-1) signaling has been proposed as a contributing factor to the development of neurodegenerative disorders including diabetic neuropathy, and delivery of exogenous IGF-1 has been explored as a treatment for Alzheimer's disease and amyotrophic lateral sclerosis. However, the role of autocrine/paracrine IGF-1 in neuroprotection has not been well established. We therefore used in vitro cell culture systems and animal models of diabetic neuropathy to characterize endogenous IGF-1 in sensory neurons and determine the factors regulating IGF-1 expression and/or affecting neuronal health. Single-cell RNA sequencing (scRNA-Seq) and in situ hybridization analyses revealed high expression of endogenous IGF-1 in non-peptidergic neurons and satellite glial cell2022-01-01T00:00:00Z2022 Mar2026-03-31T11:40:50.641Z2024-11-09T21:42:51.372ZS-EPMC89307983529871710.1007/s00018-022-04201-9