biostudies-literatureUnknown12Ma XMNational Natural Science Foundation of ChinaNatural Science Foundation of Guangdong ProvinceImmunotherapy targeting programmed death ligand-1/programmed cell death protein-1 (PD-L1/PD-1) has achieved great success in multiple cancers, but only a small subset of patients showed clinical responses. Recent evidences have shown that post-translational modification of PD-L1 protein could regulate its protein stability and interaction with cognate receptor PD-1, thereby affecting anticancer immunotherapy in several solid tumors. However, the molecular mechanisms underlying how PD-1/PD-L1 expression is regulated still remain unclear in nasopharyngeal carcinoma (NPC). Here, we found N-glycosylation of PD-L1 in NPC cells and tissues. Mechanistically, we showed that STT3A transferred N-linked glycans to PD-L1, and TGF-β1 could positively regulate STT3A expression through activating c-Jun tFrontiers in oncology815437https://www.ebi.ac.uk/biostudies/studies/S-EPMC8930841biostudies-literatureTGF-β1-Mediated PD-L1 Glycosylation Contributes to Immune Escape <i>via</i> c-Jun/STT3A Pathway in Nasopharyngeal Carcinoma.PMC8930841Liu XSu CLuo YFLi XPLu JMa XMZeng FFfalseTGF-β1-Mediated PD-L1 Glycosylation Contributes to Immune Escape <i>via</i> c-Jun/STT3A Pathway in Nasopharyngeal Carcinoma.Immunotherapy targeting programmed death ligand-1/programmed cell death protein-1 (PD-L1/PD-1) has achieved great success in multiple cancers, but only a small subset of patients showed clinical responses. Recent evidences have shown that post-translational modification of PD-L1 protein could regulate its protein stability and interaction with cognate receptor PD-1, thereby affecting anticancer immunotherapy in several solid tumors. However, the molecular mechanisms underlying how PD-1/PD-L1 expression is regulated still remain unclear in nasopharyngeal carcinoma (NPC). Here, we found N-glycosylation of PD-L1 in NPC cells and tissues. Mechanistically, we showed that STT3A transferred N-linked glycans to PD-L1, and TGF-β1 could positively regulate STT3A expression through activating c-Jun t2022-01-01T00:00:00Z20222025-04-06T19:26:04.18Z2025-04-06T19:26:04.18ZS-EPMC89308413531111710.3389/fonc.2022.815437