{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lu Q"],"funding":["China Scholarship Council","Vlaams Instituut voor Biotechnologie","Fonds Wetenschappelijk Onderzoek","Agentschap voor Innovatie door Wetenschap en Technologie"],"pagination":["e2118220119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8931322"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(11)"],"pubmed_abstract":["SignificanceChemical genetics, which investigates biological processes using small molecules, is gaining interest in plant research. However, a major challenge is to uncover the mode of action of the small molecules. Here, we applied the cellular thermal shift assay coupled with mass spectrometry (CETSA MS) to intact <i>Arabidopsis</i> cells and showed that bikinin, the plant-specific glycogen synthase kinase 3 (GSK3) inhibitor, changed the thermal stability of some of its direct targets and putative GSK3-interacting proteins. In combination with phosphoproteomics, we also revealed that GSK3s phosphorylated the auxin carrier PIN-FORMED1 and regulated its polarity that is required for the vascular patterning in the leaf."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between brassinosteroid and auxin signaling."],"pmcid":["PMC8931322"],"funding_grant_id":["na","G002121N","G009018N"],"pubmed_authors":["Ma Q","Pauwels J","Hellner J","De Smet I","Dejonghe W","Friml J","Gevaert K","Van de Cotte B","Zhang Y","Giannini C","Molina DM","Han H","Impens F","Russinova E","Xu X","Lu Q"],"additional_accession":[]},"is_claimable":false,"name":"Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between brassinosteroid and auxin signaling.","description":"SignificanceChemical genetics, which investigates biological processes using small molecules, is gaining interest in plant research. However, a major challenge is to uncover the mode of action of the small molecules. Here, we applied the cellular thermal shift assay coupled with mass spectrometry (CETSA MS) to intact <i>Arabidopsis</i> cells and showed that bikinin, the plant-specific glycogen synthase kinase 3 (GSK3) inhibitor, changed the thermal stability of some of its direct targets and putative GSK3-interacting proteins. In combination with phosphoproteomics, we also revealed that GSK3s phosphorylated the auxin carrier PIN-FORMED1 and regulated its polarity that is required for the vascular patterning in the leaf.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-25T22:59:44.892Z","creation":"2025-04-06T09:11:55.419Z"},"accession":"S-EPMC8931322","cross_references":{"pubmed":["35254915"],"doi":["10.1073/pnas.2118220119"]}}