{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fu G"],"funding":["Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project","National Key R&amp;amp;D Program of China"],"pagination":["e2119980119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8931375"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(11)"],"pubmed_abstract":["SignificanceA gene regulatory system is an important tool for the engineering of biosynthetic pathways of organisms. Here, we report the development of an inducible-ON/OFF regulatory system using a <i>malO</i> operator as a key element. We identified and modulated sequence, position, numbers, and spacing distance of <i>malO</i> operators, generating a series of activating or repressive promoters with tunable strength. The stringency and robustness are both guaranteed in this system, a maximal induction factor of 790-fold was achieved, and nine proteins from different organisms were expressed with high yields. This system can be utilized as a gene switch, promoter enhancer, or metabolic valve in synthetic biology applications. This operator-based engineering strategy can be employed for developing similar regulatory systems in different microorganisms."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["An operator-based expression toolkit for <i>Bacillus subtilis</i> enables fine-tuning of gene expression and biosynthetic pathway regulation."],"pmcid":["PMC8931375"],"funding_grant_id":["TSBICIP-KJGG-011-02","2020YFA0907800"],"pubmed_authors":["Li Y","Zhang D","Lee SY","Li D","Fu G","Yue J"],"additional_accession":[]},"is_claimable":false,"name":"An operator-based expression toolkit for <i>Bacillus subtilis</i> enables fine-tuning of gene expression and biosynthetic pathway regulation.","description":"SignificanceA gene regulatory system is an important tool for the engineering of biosynthetic pathways of organisms. Here, we report the development of an inducible-ON/OFF regulatory system using a <i>malO</i> operator as a key element. We identified and modulated sequence, position, numbers, and spacing distance of <i>malO</i> operators, generating a series of activating or repressive promoters with tunable strength. The stringency and robustness are both guaranteed in this system, a maximal induction factor of 790-fold was achieved, and nine proteins from different organisms were expressed with high yields. This system can be utilized as a gene switch, promoter enhancer, or metabolic valve in synthetic biology applications. This operator-based engineering strategy can be employed for developing similar regulatory systems in different microorganisms.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-04T20:23:41.467Z","creation":"2025-04-04T20:23:41.467Z"},"accession":"S-EPMC8931375","cross_references":{"pubmed":["35263224"],"doi":["10.1073/pnas.2119980119"]}}