{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shitikov E"],"funding":["Russian Science Foundation"],"pagination":["817024"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8931766"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["G-quadruplexes (G4s) are non-canonical DNA structures that could be considered as potential therapeutic targets for antimicrobial compounds, also known as G4-stabilizing ligands. While some of these ligands are shown <i>in vitro</i> to have a stabilizing effect, the precise mechanism of antibacterial action has not been fully investigated. Here, we employed genome-wide RNA-sequencing to analyze the response of <i>Mycobacterium smegmatis</i> to inhibitory concentrations of BRACO-19 and TMPyP4 G4 ligands. The expression profile changed (FDR < 0.05, log<sub>2</sub>FC > |1|) for 822 (515↑; 307↓) genes in <i>M. smegmatis</i> in response to BRACO-19 and for 680 (339↑; 341↓) genes in response to TMPyP4. However, the analysis revealed no significant ligand-induced changes in the expression levels "],"journal":["Frontiers in microbiology"],"pubmed_title":["Genome-Wide Transcriptional Response of <i>Mycobacterium smegmatis</i> MC<sup>2</sup>155 to G-Quadruplex Ligands BRACO-19 and TMPyP4."],"pmcid":["PMC8931766"],"funding_grant_id":["19-75-10109"],"pubmed_authors":["Shitikov E","Bodoev I","Ilina E","Bespiatykh D","Malakhova M","Zaychikova M","Klimina K","Bespyatykh J","Varizhuk A","Vedekhina T","Veselovsky V"],"additional_accession":[]},"is_claimable":false,"name":"Genome-Wide Transcriptional Response of <i>Mycobacterium smegmatis</i> MC<sup>2</sup>155 to G-Quadruplex Ligands BRACO-19 and TMPyP4.","description":"G-quadruplexes (G4s) are non-canonical DNA structures that could be considered as potential therapeutic targets for antimicrobial compounds, also known as G4-stabilizing ligands. While some of these ligands are shown <i>in vitro</i> to have a stabilizing effect, the precise mechanism of antibacterial action has not been fully investigated. Here, we employed genome-wide RNA-sequencing to analyze the response of <i>Mycobacterium smegmatis</i> to inhibitory concentrations of BRACO-19 and TMPyP4 G4 ligands. The expression profile changed (FDR < 0.05, log<sub>2</sub>FC > |1|) for 822 (515↑; 307↓) genes in <i>M. smegmatis</i> in response to BRACO-19 and for 680 (339↑; 341↓) genes in response to TMPyP4. However, the analysis revealed no significant ligand-induced changes in the expression levels ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-04-08T18:24:00.478Z","creation":"2024-10-17T20:07:05.29Z"},"accession":"S-EPMC8931766","cross_references":{"pubmed":["35308348"],"doi":["10.3389/fmicb.2022.817024"]}}