biostudies-literatureShitikov ERussian Science Foundation817024https://www.ebi.ac.uk/biostudies/studies/S-EPMC8931766biostudies-literatureUnknown13G-quadruplexes (G4s) are non-canonical DNA structures that could be considered as potential therapeutic targets for antimicrobial compounds, also known as G4-stabilizing ligands. While some of these ligands are shown <i>in vitro</i> to have a stabilizing effect, the precise mechanism of antibacterial action has not been fully investigated. Here, we employed genome-wide RNA-sequencing to analyze the response of <i>Mycobacterium smegmatis</i> to inhibitory concentrations of BRACO-19 and TMPyP4 G4 ligands. The expression profile changed (FDR < 0.05, log₂FC > |1|) for 822 (515↑; 307↓) genes in <i>M. smegmatis</i> in response to BRACO-19 and for 680 (339↑; 341↓) genes in response to TMPyP4. However, the analysis revealed no significant ligand-induced changes in the expression levels Frontiers in microbiologyGenome-Wide Transcriptional Response of <i>Mycobacterium smegmatis</i> MC²155 to G-Quadruplex Ligands BRACO-19 and TMPyP4.PMC893176619-75-10109Shitikov EBodoev IIlina EBespiatykh DMalakhova MZaychikova MKlimina KBespyatykh JVarizhuk AVedekhina TVeselovsky VfalseGenome-Wide Transcriptional Response of <i>Mycobacterium smegmatis</i> MC²155 to G-Quadruplex Ligands BRACO-19 and TMPyP4.G-quadruplexes (G4s) are non-canonical DNA structures that could be considered as potential therapeutic targets for antimicrobial compounds, also known as G4-stabilizing ligands. While some of these ligands are shown <i>in vitro</i> to have a stabilizing effect, the precise mechanism of antibacterial action has not been fully investigated. Here, we employed genome-wide RNA-sequencing to analyze the response of <i>Mycobacterium smegmatis</i> to inhibitory concentrations of BRACO-19 and TMPyP4 G4 ligands. The expression profile changed (FDR < 0.05, log₂FC > |1|) for 822 (515↑; 307↓) genes in <i>M. smegmatis</i> in response to BRACO-19 and for 680 (339↑; 341↓) genes in response to TMPyP4. However, the analysis revealed no significant ligand-induced changes in the expression levels 2022-01-01T00:00:00Z20222026-04-08T18:24:00.478Z2024-10-17T20:07:05.29ZS-EPMC89317663530834810.3389/fmicb.2022.817024