biostudies-literatureTu CFInstitute of Biomedical SciencesMinistry of Science and Technology, TaiwanAcademia Sinica, Taiwan21https://www.ebi.ac.uk/biostudies/studies/S-EPMC8932202biostudies-literatureUnknown24(1)<h4>Background</h4>We recently showed that fucosyltransferase 8 (FUT8)-mediated core fucosylation of transforming growth factor-β receptor enhances its signaling and promotes breast cancer invasion and metastasis. However, the complete FUT8 target glycoproteins and their downstream signaling networks critical for breast cancer progression remain largely unknown.<h4>Method</h4>We performed quantitative glycoproteomics with two highly invasive breast cancer cell lines to unravel a comprehensive list of core-fucosylated glycoproteins by comparison to parental wild-type and FUT8-knockout counterpart cells. In addition, ingenuity pathway analysis (IPA) was performed to highlight the most enriched biological functions and signaling pathways mediated by FUT8 targets. Novel FUT8 target glycoproteiBreast cancer research : BCRQuantitative glycoproteomics analysis identifies novel FUT8 targets and signaling networks critical for breast cancer cell invasiveness.PMC8932202MOST 110-2320-B-001-019-MY3IBMS-CRC110-P04AS-KPQ-111-KNTMOST 107-2320-B-001-015-MY3MOST 109-2320-B-001-012-MY3AS-GC-111-L04Li LHLi FAYang RBTu CFfalseQuantitative glycoproteomics analysis identifies novel FUT8 targets and signaling networks critical for breast cancer cell invasiveness.<h4>Background</h4>We recently showed that fucosyltransferase 8 (FUT8)-mediated core fucosylation of transforming growth factor-β receptor enhances its signaling and promotes breast cancer invasion and metastasis. However, the complete FUT8 target glycoproteins and their downstream signaling networks critical for breast cancer progression remain largely unknown.<h4>Method</h4>We performed quantitative glycoproteomics with two highly invasive breast cancer cell lines to unravel a comprehensive list of core-fucosylated glycoproteins by comparison to parental wild-type and FUT8-knockout counterpart cells. In addition, ingenuity pathway analysis (IPA) was performed to highlight the most enriched biological functions and signaling pathways mediated by FUT8 targets. Novel FUT8 target glycoprotei2022-01-01T00:00:00Z2022 Mar2025-04-26T18:31:30.425Z2025-04-06T15:49:28.44ZS-EPMC89322023530392510.1186/s13058-022-01513-3