biostudies-literatureUnknown8(11)Niu MThe processing of stress responses involves brain-wide communication among cortical and subcortical regions; however, the underlying mechanisms remain elusive. Here, we show that the claustrum (CLA) is crucial for the control of stress-induced anxiety-related behaviors. A combined approach using brain activation mapping and machine learning showed that the CLA activation serves as a reliable marker of exposure to acute stressors. In TRAP2 mice, which allow activity-dependent genetic labeling, chemogenetic activation of the CLA neuronal ensemble tagged by acute social defeat stress (DS) elicited anxiety-related behaviors, whereas silencing of the CLA ensemble attenuated DS-induced anxiety-related behaviors. Moreover, the CLA received strong input from DS-activated basolateral amygdala neuroScience advanceseabi6375https://www.ebi.ac.uk/biostudies/studies/S-EPMC8932664biostudies-literatureClaustrum mediates bidirectional and reversible control of stress-induced anxiety responses.PMC8932664Seiriki KOhkubo JTanuma MYokoyama RTanabe WMiyaji KOkuno HAgo YInoue KINakazawa TNagayasu KSeo KKuwaki TKaneko SYamaguchi SHayashida MTakada MIgarashi HUeno HYamanaka ANiu MKasai AHashimoto HfalseClaustrum mediates bidirectional and reversible control of stress-induced anxiety responses.The processing of stress responses involves brain-wide communication among cortical and subcortical regions; however, the underlying mechanisms remain elusive. Here, we show that the claustrum (CLA) is crucial for the control of stress-induced anxiety-related behaviors. A combined approach using brain activation mapping and machine learning showed that the CLA activation serves as a reliable marker of exposure to acute stressors. In TRAP2 mice, which allow activity-dependent genetic labeling, chemogenetic activation of the CLA neuronal ensemble tagged by acute social defeat stress (DS) elicited anxiety-related behaviors, whereas silencing of the CLA ensemble attenuated DS-induced anxiety-related behaviors. Moreover, the CLA received strong input from DS-activated basolateral amygdala neuro2022-01-01T00:00:00Z2022 Mar2025-04-04T03:20:21.846Z2025-04-04T03:20:21.846ZS-EPMC89326643530285310.1126/sciadv.abi6375