biostudies-literatureOliviero GDanmarks GrundforskningsfondVillum FondenDanish Agency for Science and Higher Educatione73524https://www.ebi.ac.uk/biostudies/studies/S-EPMC8933006biostudies-literatureUnknown11Temporal molecular changes in ageing mammalian organs are of relevance to disease aetiology because many age-related diseases are linked to changes in the transcriptional and epigenetic machinery that regulate gene expression. We performed quantitative proteome analysis of chromatin-enriched protein extracts to investigate the dynamics of the chromatin proteomes of the mouse brain, heart, lung, kidney, liver, and spleen at 3, 5, 10, and 15 months of age. Each organ exhibited a distinct chromatin proteome and sets of unique proteins. The brain and spleen chromatin proteomes were the most extensive, diverse, and heterogenous among the six organs. The spleen chromatin proteome appeared static during the lifespan, presenting a young phenotype that reflects the permanent alertness state and impeLifeDistinct and diverse chromatin proteomes of ageing mouse organs reveal protein signatures that correlate with physiological functions.PMC8933006DNRF #825072-00007B7292Schwammle VRogowska-Wrzesinska AKovalchuk SJensen ONOliviero GfalseDistinct and diverse chromatin proteomes of ageing mouse organs reveal protein signatures that correlate with physiological functions.Temporal molecular changes in ageing mammalian organs are of relevance to disease aetiology because many age-related diseases are linked to changes in the transcriptional and epigenetic machinery that regulate gene expression. We performed quantitative proteome analysis of chromatin-enriched protein extracts to investigate the dynamics of the chromatin proteomes of the mouse brain, heart, lung, kidney, liver, and spleen at 3, 5, 10, and 15 months of age. Each organ exhibited a distinct chromatin proteome and sets of unique proteins. The brain and spleen chromatin proteomes were the most extensive, diverse, and heterogenous among the six organs. The spleen chromatin proteome appeared static during the lifespan, presenting a young phenotype that reflects the permanent alertness state and imp2022-01-01T00:00:00Z2022 Mar2025-04-04T14:49:41.835Z2025-04-04T14:49:41.835ZS-EPMC89330063525909010.7554/eLife.73524